Recombinant Human SKP1/FBXO7 Complex Protein, CF

R&D Systems | Catalog # E3-525

R&D Systems
Discontinued Product
E3-525 has been discontinued. View all SKP1/FBX07 Complex products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

P63208, Q9Y3I1

Applications

Bioactivity
View all SKP1/FBX07 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/FBX07 Complex protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

21 kDa (SKP1), 63 kDa (FBXO7)

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-525
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage

Background: SKP1/FBX07 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases.  FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20 and other mitochondrial proteins.  Evidence from a Drosophila Parkin-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings.  This recombinant protein complex consists of His-tag SKP1 and FLAG-tag FBXO7.

References

  1. Burchell V.S., et al. (2013) Nat. Neurosci.  16: 1257
  2. Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm.  342: 1022
  3. Hsu J.M., et al. (2004) J. Biol. Chem.  279: 32592
  4. Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci.  18: 715
  5. Stott S.R. W., et al. (2019) J. Pathol.  249: 241

Long Name

S-phase Kinase Associated Protein 1/F-Box Only Protein 7

UniProt

P63208, Q9Y3I1

Product Documents for Recombinant Human SKP1/FBXO7 Complex Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SKP1/FBXO7 Complex Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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