Tricine

Tocris Bioscience | Catalog # 5227

Electrophoresis and SDS-PAGE buffer
Tocris Bioscience
Discontinued Product
5227 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Electrophoresis and SDS-PAGE buffer
View all Buffers, Chelators and Reagents »

Product Description

Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.

Product Specifications for Tricine

Molecular Weight

179.17

Formula

C6H13NO5

Storage

Store at RT

Purity

≥99%

Chemical Name

N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine

CAS Number

5704-04-1

PubChem ID

79784

InChI Key

SEQKRHFRPICQDD-UHFFFAOYSA-N

SMILES

OCC(CO)(CO)NCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 17.92 100

Preparing Stock Solutions for Tricine

The following data is based on the product molecular weight 179.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.58 mL 27.91 mL 55.81 mL
5 mM 1.12 mL 5.58 mL 11.16 mL
10 mM 0.56 mL 2.79 mL 5.58 mL
50 mM 0.11 mL 0.56 mL 1.12 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for Tricine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Tricine

For research use only

Customer Reviews for Tricine (1)

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  • Tris-glycine protein gel produced using Tricine
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/06/2019
    Tris-glycine gel formation for electrophoresis carried out by tricine. Gives better resolution for low molecular weight protein. Highly suggest using this buffer for separation of proteins that are small.
    Tricine 5227

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