Using Antibody Arrays for Assessing the Integration of Multiple Signaling Pathways and Off-Target Inhibitors

by Greta Wegner, Erin Eleria, Jackie Ernst, & Amy James.

Scientific Meeting Posters

The Problem: Time-consuming Western blots

  • Assessing phosphorylation changes in multiple interrelated pathways
  • Performing pharmacological screens in a timely manner
  • Identifying off-target pharmacological effects

The Solution: Proteome Profiler™ Phospho-Antibody Arrays

  • Easier to perform than a Western blot
  • Uncover new relationships between signaling pathways
  • Identify off-target pharmacological responses that can confound results and waste time
  • Collect data from the array membrane in the same manner as an immunoblot

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SDS-PAGE Analysis of Recombinant Human COL1A1, COL2A1, and COL3A1