Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 27311
View all IL-2 R beta Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869

Specificity

Detects human IL-2 R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IL-2 R alpha, recombinant mouse IL-2 R beta, rhIL-2 R gamma, rhIL-15 R alpha, or rhIL-21 R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑2 R beta Antibody

Detection of Human IL‑2 R beta by Simple WesternTM.

Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IL‑2 R beta at approximately 98 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB2241). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Applications for Human IL‑2 R beta Antibody

Application
Recommended Usage

Simple Western

25 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑2 R beta (Catalog # 224-2B)

Reviewed Applications

Read 1 review rated 4 using MAB2241 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain are required for IL-15 mediated signaling.

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I transmembrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Gene Symbol

IL2RB

UniProt

NP_000869

Additional IL-2 R beta Products

Product Documents for Human IL‑2 R beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑2 R beta Antibody

For research use only

Citations for Human IL‑2 R beta Antibody

Customer Reviews for Human IL‑2 R beta Antibody (1)

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  • Human IL-2 R beta Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/17/2019
    Human IL‑2 R beta Antibody MAB2241

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