Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91183

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

2.1 pg/mL

Assay Range

3.1 - 200 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-12p70 positive sample. There was no cross reactivity or interference detected.

Description

The human IL-12 p70 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-12 p70

精度

Intra-Assay Precision (Precision within an assay) CV% 3.00%

Inter-Assay Precision (Precision between assays) CV% 4.80%

Recovery for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 79% to 118% with an overall mean recovery of 103%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

ELISA: Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-91183]

ELISA: Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-91183]

ELISA: Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-91183] - Data for Human IL-12 (p70) ELISA Kit

Kit Contents for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

  • 1 aluminum pouch with a Microwell Plate (12 strips with 8 wells each) coated with monoclonal antibody to human IL-12 p70/IL-12A
  • 1 bottle (50 mL) Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • 1 vial (12 mL) Sample Diluent
  • 1 vial (15 mL) Stop Solution (1M Phosphoric acid)
  • 1 vial (15 mL) Substrate Solution (tetramethyl-benzidine)
  • 1 vial (150 µL) Streptavidin-HRP
  • 1 vial (5 mL) Assay Buffer Concentrate 20x (PBS with 1% Tween 20 10% BSA)
  • 1 vial (70 µL) Biotin-Conjugate anti-human IL-12 p70/IL-12A monoclonal antibody
  • 1 vial Control high (lyophilized)
  • 1 vial Control low (lyophilized)
  • 2 vials human IL-12 p70/IL-12A Standard lyophilized (400 pg/mL upon reconstitution)
  • 4 Adhesive Films

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12 p70

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

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FAQs for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

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    • Q: Does the human IL-12 polyclonal antibody (Catalog # AB-219-NA) recognize IL-12 p70?
      A: In Western blots, this antibody recognizes the p70 heterodimer as well as the p40 and p35 subunits.
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