Mouse Complement Factor H Antibody

Detection of Mouse Complement Factor H by Western Blot.

Western blot shows mouse plasma and lysate of mouse liver tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor H Monoclonal Antibody (Catalog # MAB4999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Complement Factor H at approximately 200 and 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Complement Factor H by Immunocytochemistry/Immunofluorescence

Histologic evidence of AP activation in experimental MN. Glomerular deposition of properdin (A), factor H (B), and C4d (C) was evaluated by indirect immunofluorescence staining of frozen kidney sections from alpha 3NC1-immunized D1 mice and adjuvant-immunized control mice. Properdin and factor H staining along the capillary loops was found in alpha 3NC1-immunized D1 mice, but not in adjuvant-immunized mice. Mesangial C4d staining was found in all mice, while weaker segmental staining for C4d along capillary loops was also present in alpha 3NC1-immunized mice. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29988342), licensed under a CC-BY license. Not internally tested by R&D Systems.