Mouse IL-6 PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IL-6

IL-6 (Interleukin-6), also known as Interferon beta 2, is a secreted, 24-30 kDa member of the IL-6 family of proteins. In mouse, mature IL-6 is a highly glycosylated, monomeric polypeptide that is 187 amino acids (aa) in length, and shares 85% and 40% aa identity with rat and human IL-6, respectively. IL-6 expression is induced in a highly diverse group of cells including vascular and visceral smooth muscle cells, T helper cells, fibroblasts, astrocytes, endothelial cells, neurons, monocytes, microglia, pancreatic islet alpha -cells, osteoblasts, keratinocytes and white adipocytes. The receptor for IL-6 is composed of two distinct type I transmembrane glycoproteins, an 80-85 kDa ligand-binding IL-6R alpha subunit, and two signal-transducing 130-135 kDa gp130 subunits. Notably, the IL-6 system seems to exist as one of two states; there is a "classic signaling" state where IL-6 interacts with the transmembrane forms of both IL-6R alpha and gp130 in a signaling complex, and an IL-6 "trans-signaling" state, where IL-6 is bound to soluble IL-6R alpha in a circulating complex that subsequently interacts with transmembrane gp130. The "classic signaling" complex occurs on select cells such as hepatocytes, neutrophils and monocytes. The outcome of "classic signaling" is typically anti-inflammatory, promoting hepatocyte and pancreatic proliferation and regeneration, plus hepatic acute phase protein production. "Trans-signaling", by contrast, is very widespread, as a soluble IL-6R alpha :IL-6 complex can go almost everywhere and interact with the ubiquitously-expressed gp130. Relative to "classic signaling" however, this type of signaling is typically pro-inflammatory, and results in endothelial and smooth muscle cell activation, an inhibition of Treg differentiation, and a mononuclear cell infiltration into inflammatory environments.

Product Datasheets

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