Rat IgG1 PE-conjugated Antibody

  (1 citations)     
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Product Details
Citations (1)
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  • Specificity
    Selected for use as rat IgG1 isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG1 Clone # 43414
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Rat IgGControl by Flow Cytometry RPMI 8226 human multiple myeloma cell line was stainedwith Rat Anti-Human Syndecan-1/CD138 PE-conjugated Monoclonal Antibody (Catalog #FAB2780P,filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody(Catalog # IC005P, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G1
  • Entrez Gene IDs:
    101867415 (Cynomolgus Monkey)
  • Alternate Names:
    IgG1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Isotype Control FAQs
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 40  
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Rat IgG1 APC-conjugated Isotype Control

Ctrl IC005A 1
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Rat IgG1 Fluorescein‑conjugated Control Antibody

Ctrl IC005F 2
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Rat IgG1 Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC005G
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Rat IgG1 Alexa Fluor® 750-conjugated Antibody

Ctrl IC005S
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Rat IgG1 Alexa Fluor® 594-conjugated Antibody

Ctrl IC005T
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Rat IgG1 Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC005N
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Rat IgG1 Alexa Fluor® 405-conjugated Antibody

Ctrl IC005V
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Rat IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC005R
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Rat IgG1 Isotype Control

Ctrl MAB005R
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