Discontinued Product
4892-010-02 has been discontinued.
View all Cell Viability/Proliferation Assays products.
Key Product Details
Features
10X Calcein AM Dilution/Wash (DW) Buffer is a component of Calcein AM Cell Viability Assay Kit.
Product Summary for 10X Calcein AM DW Buffer
How is 10X Calcein AM Dilution/Wash Buffer Used?
10X Calcein AM DW Buffer features a 200 mL presentation. To obtain a working stock, dilute 10X Calcein AM DW Buffer before use by adding 5 mL of 10X Calcein AM DW buffer to 45 mL of deionized sterile H20. This working stock is enough for one 96-well microplate.
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
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Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Product Documents for 10X Calcein AM DW Buffer
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
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Customer Reviews for 10X Calcein AM DW Buffer (2)
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Verified Customer | Posted 04/25/2018Used along with calcein AM solution
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Verified Customer | Posted 04/04/2018
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