10X Calcein AM DW Buffer
10X Calcein AM DW Buffer Summary
How is 10X Calcein AM Dilution/Wash Buffer Used?
10X Calcein AM DW Buffer features a 200 mL presentation. To obtain a working stock, dilute 10X Calcein AM DW Buffer before use by adding 5 mL of 10X Calcein AM DW buffer to 45 mL of deionized sterile H20. This working stock is enough for one 96-well microplate.
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
For research use only. Not for diagnostic use.
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Used along with calcein AM solution