10X Calcein AM DW Buffer

Catalog # Availability Size / Price Qty
4892-010-02
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10X Calcein AM DW Buffer Summary

10X Calcein AM Dilution/Wash (DW) Buffer is a component of Calcein AM Cell Viability Assay Kit.

How is 10X Calcein AM Dilution/Wash Buffer Used?

10X Calcein AM DW Buffer features a 200 mL presentation. To obtain a working stock, dilute 10X Calcein AM DW Buffer before use by adding 5 mL of 10X Calcein AM DW buffer to 45 mL of deionized sterile H20. This working stock is enough for one 96-well microplate.

What is Calcein AM?

Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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