10X Calcein AM DW Buffer

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Reviews (2)

10X Calcein AM DW Buffer Summary

10X Calcein AM Dilution/Wash (DW) Buffer is a component of Calcein AM Cell Viability Assay Kit.

How is 10X Calcein AM Dilution/Wash Buffer Used?

10X Calcein AM DW Buffer features a 200 mL presentation. To obtain a working stock, dilute 10X Calcein AM DW Buffer before use by adding 5 mL of 10X Calcein AM DW buffer to 45 mL of deionized sterile H20. This working stock is enough for one 96-well microplate.

What is Calcein AM?

Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Store the unopened product at room temperature. Do not use past expiration date.


For research use only. Not for diagnostic use.

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10X Calcein AM DW Buffer
By Anonymous on 04/25/2018

Used along with calcein AM solution

10X Calcein AM DW Buffer
By Leslie Priddy on 04/04/2018