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R&D Systems® ELISA Kits

R&D Systems® ELISA Kits are the most trusted, most published ELISAs on the market with a menu of 700 target analytes spanning 12 species. Our ELISAs perform as expected every time because of our high-quality manufacturing and performance standards. Quantikine™ ELISA kits are ready to use. DuoSet™ Development Kits allow for more flexibility.

R&D Systems ELISA Kits

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Ella

Immunoassays for COVID-19 Research

Profile the immune response to COVID-19 infection or vaccine with our large portfolio of immunoassays including single and multiplex immunoassays. Measure anti-SARS-CoV-2 IgG antibody responses with our quantitative SARS-CoV-2 IgG ELISA Kit.

 

ELISA Kits for CTLA-4 and Immune Checkpoint Research

CTLA-4 plays a dual role in autoimmunity and Immune checkpoint research. The R&D Systems Human Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) High Sensitivity ELISA is sensitive, specific, and provides high-quality data so you don’t have to worry about repeating experiments.

 

Contents

Ready to use ELISA Kits - Quantikine

Quantikine ELISA kits are available in a range of formats for measuring intracellular and extracellular proteins. Our master calibrated Quantikine assays ensure that our kits perform consistently over time and are optimized to mitigate matrix effects and avoid false-positive results.

Colorimetric Sandwich ELISA Kits
Complete ready-to-use colorimetric sandwich ELISA kits. Our Quantikine ELISA kits are the most published ELISAs on the market, they are the ELISA Industry Gold Standard.

High Sensitivity Colorimetric Sandwich ELISA Kits
ELISA kits developed with greater sensitivity for quantification of low level targets in your sample.

QuicKit™ Colorimetric Sandwich ELISA Kits
QuicKit ELISAs have a fast, simplified protocol that produces results in 90 minutes with just one wash step, all without compromising quality.

In Vitro Diagnostic (IVD) Colorimetric ELISA Kits
ELISA kits that are 510(k) cleared for IVD use. These highly documented and validated kits are provided ready to use with discrete standards for ease of use.

QuantiGlo™ Chemiluminescent Sandwich ELISA Kits
Chemilluminescent ELISA kits utilize a luminescent substrate that allows for measuring samples over a broad dynamic range.

Parameter Colorimetric Competitive ELISA Kits
These competitive ELISA kits are designed to measure small molecules and hormones.

Protein A ELISA Kits
ELISA kits for accurate detection of Protein A residuals in bioprocessing applications.

ELISA Kits for Unique Model Organisms
Colorimetric sandwich ELISA kits designed and optimized for model organisms including non-human primate, mouse, and rat, among others.

Custom Assay Services
Customized immunoassays for new, novel targets or modification of existing immunoassays for your research platform.

View All Ready to Use ELISA Kits
Search our entire menu of coated ELISA kits.

 

 

Build your ELISA - DuoSet

DuoSet ELISA Development Kits contain matched antibody pairs and the basic components required to develop an immunoassay. They offer an economical alternative to buying separate antibodies and proteins and optimizing your own assay.

ELISA Development Systems
DuoSet assays contain the basic components required to develop a sandwich ELISA to measure recombinant or natural proteins. The DuoSet development assays offer an economical alternative to other R&D Systems ELISA kits for experienced ELISA-users.

IC (Intracellular) ELISA Development Systems
Offers a fast and sensitive method to detect and quantify intracellular factors in cell lysate samples.

IC (Intracellular) Phospho-specific ELISA Development Systems
Measure phosphorylated intracellular molecules in cell lysates by sandwich ELISA.

Supplemental ELISA/Assay Products
Materials and reagents to run your DuoSet ELISA kit. Microplates, wash buffers, diluents, detection reagents, stop solution, sample collection kits, and more.

View All DuoSet Development Systems
Search the largest menu of kits for building your own ELISA.

Custom Assay Services
Customized immunoassays for new, novel targets or modification of existing immunoassays for your research platform.

 

 

Automated ELISA Platform - Ella™

Meet Ella, your immunoassay problem solver. Her Simple Plex™ assays are fully automated, multi-analyte immunoassays. And, the coolest part? They’re powered by antibodies from R&D Systems.

View Automated ELISA Platform, Ella
Ella’s multi-sample, ready to go cartridges let you run up to 4 immunoassays per sample, all at once. On top of that, Ella just uses 25 μL of sample! Setup only takes 5 minutes, and she'll have your data ready in 60 minutes. Hands-free.

Simple Plex Assays Menu on ProteinSimple.com
Simple Plex™ assays run on Ella - they're like mini, automated ELISAs. Single plex, multiplex, multianalyte and customizable cartridge options.

 

Introduction to ELISAs

What is an ELISA?
Discover the different types of ELISAs as well as when and why to use them

What is the importance of ELISA Controls?
Why to use positive and negative controls in your ELISA

ELISA Sample Preparation & Collection Guide
General sample collection guidelines for various sample types

10 Best ELISA Practices and Techniques
ELISA tips to get the best results

How to Analyze ELISA Data
Creating a standard curve and calculating sample concentrations

Troubleshooting Guide: ELISA Development
Possible solutions for when your ELISA results don’t look right

 

 

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How to Run an R&D Systems Quantikine ELISA

This video is a general step-by-step guide to running an R&D Systems Quantikine ELISA kit. Quantikine ELISA kits are highly validated, ready-to-use ELISAs that come with all reagents needed to run the assay. Quankitine ELISAs are the most published ELISA kits and recognized as the gold standard ELISA. In this video we are running a Human Leptin Receptor ELISA Kit to determine Leptin Receptor concentrations in serum samples and controls.

How to Run an R&D Systems Quantikine ELISA
How to Run an R&D Systems Quantikine ELISA

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How to Run an R&D Systems DuoSet ELISA

This video is a general step-by-step guide to running an R&D Systems DuoSet ELISA kit. DuoSet ELISA Development Systems contain the basic components required to develop a sandwich ELISA, including matched antibody pairs, standard, and streptavidin HRP. In this video we are running a Human TNF-alpha DuoSet ELISA kit and cover all steps from coating to reading a plate.

How to Run an R&D Systems DuoSet ELISA
How to Run an R&D Systems DuoSet ELISA

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How to Run an R&D Systems Quantikine QuicKit

This video is a general step-by-step guide for running an R&D Systems QuicKit ELISA. R&D Systems QuicKit ELISAs offer a shortened protocol of under 90 minutes with a single wash step, for the detection of proteins in various sample types. QuicKit ELISAs use plates precoated with an anti-tag antibody. In this video we are running a Human TNF-alpha QuicKit ELISA to determine human TNF-alpha concentrations in serum and cell culture supernates.

How to Run an R&D Systems Quantikine QuicKit
How to Run an R&D Systems Quantikine QuicKit

ELISA Recognition Awards

R&D Systems is honored to be recognized for multiple recent awards. For more than three decades, we have strived to provide our customers with the best quality products we can produce. We take great pride in knowing that our products allow our customers to make major advances in life sciences research. Learn more about our R&D Systems ELISA quality story.

COVID-19 Innovation Award, 2021

COVID-19 Innovation Award:
Creation of new assays, antibodies, and proteins to aid in COVID-19 research, 2021.

Most cited ELISA award, 2020

Bioz Top StarTM:
Most citations in ELISA category, 2020.

 

 

Highest reviewed ELISA kits, 2020

Researcher’s Choice:
Based on researchers who used R&D Systems ELISA kits, 2020.

 

Most cited ELISA award, 2019

Bioz Top StarTM:
Most citations in ELISA category, 2019.

 

 

Most cited ELISA award, 2018

ELISA Company of the Year:
Most ELISA kit citations, 2018.

ELISA kits with the highest reviews, 2018

Researcher’s Choice:
Based on researchers who used R&D Systems ELISA kits, 2018.

 

ELISA Articles and Additional Resources

Featured Content

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NEW! SARS-CoV-2 Variant Inhibitor Screening Kit

NEW! SARS-CoV-2 Variant Inhibitor Screening Kit

Screen samples for Spike blocking antibodies. Wild Type, Alpha, Beta, Gamma, and Delta Spike Variants. All in one kit!

Learn More

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Immunoassays for COVID-19 Research

Get the assays you need for fast, accurate detection of key cytokines and antibodies triggered by viral infections like COVID-19

See all Coronavirus Assays

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ELISA Tips and Tricks Poster

ELISA Tips and Tricks Poster

Bio-Techne has over 30 years of ELISA experience and has compiled the top 10 best practices and techniques for your reference.

Request ELISA Tips and Tricks Poster

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The ELISA Guide

ELISA guide handbook

A clear and easy guide to ELISAs

Download ELISA guide »

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Avoid False Positive ELISA Data

Avoid False Positive ELISA Data

This app note will help you generate data you can trust.

Download the ELISA app note

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Quantify IFN-gamma with Confidence

Quantify IFN-gamma with Confidence

IFN-gamma release information and assays. From single to multiplex assays we have the solutions to fit all research needs.

Explore IFN-gamma Immunoassays

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Luminex Assay Users Guide

Luminex Assay Users Guide

You will learn about Luminex sample preparations, protocols, standard procedures, data analysis, and more!

Download The Guide

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