Simple Plex Immunoassays
Automated Immunoassay System for Precise and Reproducible Results
What is Simple Plex Technology?
Simple Plex Technology combines the Ella Automated Immunoassay System, with ready-to-run Simple Plex Assays for unmatched precision and consistency. Together, they deliver fast, hands-free results you can rely on—with the ability to measure up to eight analytes in less than 90 minutes.
Hands-free Automation- results in 90 minutes
Simple Plex Immunoassays leverage microfluidic cartridges to obtain high-sensitivity biomarker detection, combining precision and efficiency within a closed, automated platform. This system relies on microfluidic technology to miniaturize and streamline traditional immunoassay workflows, allowing for multiplexed analysis with minimal sample volume. By incorporating pre-loaded reagents and automated handling, Simple Plex immunoassays offer reduced variability and faster results compared to conventional methods like ELISA.
- Sandwich immunoassay occurs within Glass Nano Reactors (GNRs)
- 2-3 GNRs within each channel provide duplicate/triplicate results per sample
Microfluidic Sandwich ELISA
- Sample is routed through a microfluidic circuitry, with synchronized release of assay reagents
- Cartridges are pneumatically operated, pumping liquids from different reservoirs throughout the assay run
Single Analyte Immunoassays
Single Plex Assays allow you to quantify 1 analyte in a single microfluidic channel. Single Plex Simple Plex cartridges are available in 16, 32, and 72 sample configurations, with built-in triplicate measurements. When you need high-quality data from your sample and high throughput, the 72x1 cartridge offers a fast and scalable option.
Multi-analyte Immunoassays
Multianalyte assays allow you to quantify 2, 3, or 4 target analytes in parallel. Each microfluidic channel has a unique immunoassay sandwich, consisting of high-quality capture and detection antibodies. Multianalyte Simple Plex cartridges are available in 16 and 32 sample configurations, with built-in triplicate measurements for every analyte. When you need high-quality data from your sample and high throughput, the 32x2, 32x3, and 32x4 cartridges offer flexible options.
Multiplex Immunoassays
Multiplex assays allow you to quantify 5, 6, 7, or 8 analytes in duplicate. Each microfluidic channel contains two spatially separated capture antibodies so you can quantify up to 8 target analytes per sample. This cost-effective assay allows you to maximize the data that you can extract from each of 32 samples in 90 minutes or less. Generate up to 256 reportable results from each multiplex assay.
Get Consistent Results
Robust and reproducible answers across multiple users and multiple sites
- Built-in standard curve minimizes operator error
- Automation minimizes user variability
- Microfluidics enable small sample volumes
- Consistent assays with CV <12%
Multi-site testing (all results histogram). Testing across 3 sites, 11 users and 9 Ella instruments. 4 different assays were processed for CCL2, IL-6, TNF alpha, and VEGF-A. Eight unique serum samples with 2 controls for a total of 704 data points.
Broad Dynamic Range- Detect More
Using fluorescent readout, Simple Plex immunoassays on Ella delivers better sensitivity compared to traditional colorimetric ELISA kits allowing for sensitive detection and up to 4+ logs of dynamic range.
Broad Dynamic Range- Minimizes Sample Dilution
The 4-log dynamic range enables accurate quantification of a wide range of analyte concentrations within a single run, minimizing the need for repeat measurements with multiple dilutions. This also reduces overall sample volume requirements.
Lot-to-lot Consistency : Reproducible & Reliable
Stringent QC for each lot of cartridge creates repeatable results for longitudinal sample testing.
Fully Validated Immunoassay Solution
Simple Plex Assay validation criteria to help ensure robust, reproducible, and validated results.
| Linearity- is a measure of accuracy with diluted samples. In Simple Plex Assays, all sample types are optimized for linear dilutions through the standard curve range. |
| Specificity- or true negative rate, is confidence in a negative test result. Immunoassays are subject to several potential interferences from components in complex samples like serum or plasma. Simple Plex Assays are rigorously tested to ensure the correct intended analyte is measured. |
| Recovery- or spike recovery, is a measurement of accuracy. A spike recovery study provides an estimate of how much of the available analyte is measurable in the assay and if there may be sample components interfering with that measurement. Simple Plex Assays are optimized for recovery at three different spike concentrations (low, mid, high) and for every validated sample type. |
| Precision- Consistency, or reproducibility, of a method measures precision. Simple Plex Assays ensure lot-to-lot consistency, enabling long-term research studies. This, combined with our stringent manufacturing and quality control measures, helps ensures reliable and reproducible results between kit lots and prevents sample value drift. |
ELISA correlated
Correlations between R&D Systems immunoassay solutions allows you to seamlessly move between Quantikine ELISA Kits, High Performance Luminex Assays and Simple Plex Assays on Ella.
Dr. Melissa Munroe, M.D., Ph.D.
Research Associate, Oklahoma Medical Research Foundation (OMRF)
“We needed assays that were not only excellent at conserving our limited sample volumes but would allow us to test a lot of markers quickly and efficiently. The Luminex and Simple Plex assays proved to be the ideal choice in this case, giving us a level of accuracy and reproducibility that we’ve been unable to get with other platforms.”
Dr. Zhong Youjia
Assistant Professor, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore
“With Ella, we can worry less about manpower and time constraints. It’s convenient, quick, and automated—perfect for pediatric studies with limited sample volume.”
Dr. John Canfield, PhD
Associate Director of Lab Sciences, Immunologix Laboratories
“The Ella system is a valuable tool for supporting our clients’ clinical programs. It’s robust, reliable, and helps us deliver high-quality results faster and with greater confidence.”
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