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Primary Antibodies, Secondary Antibodies, and Isotype Controls

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Primary Antibodies, Secondary Antibodies, and Isotype Controls

An antibody is a Y shaped protein that is secreted by B cells in the immune system and recognizes a specific protein or molecule. Due to the highly specific nature of antibodies, they can be used as powerful tools for research to detect targets of interest. Over the years a variety of techniques and applications have been developed using antibodies. These include Immunoassays, Flow Cytometry, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blotting, and more.

R&D Systems offers a wide range of antibodies to cytokines, adhesion molecules, proteases, neurotrophic factors, stem cell factors, signal transduction molecules, and developmental proteins. Monoclonal and polyclonal primary antibodies are available conjugated to fluorescent or enzymatic labels or unconjugated. With over 26,000 antibody products validated in 25+ species and 15+ applications, our antibodies fit into all workflows. Complete your experimental design with secondary antibodies and isotype control antibodies.

Explore Complete Catalog of Antibodies

 

View R&D Antibody Portfolio at A Glance

Choose from a high-performance portfolio so you can focus on the science. Find your antibody!

 

View R&D Antibody Portfolio at A Glance

 

Contents

Primary Antibodies

Primary antibodies are used to detect a wide range of targets in variety of applications. R&D Systems offers an extensive catalog of primary antibodies to take your research to the next level. We offer traditional monoclonal antibodies and polyclonal antibodies as well as recombinant antibodies. We provide multiple size options from our sample size to test an antibody to our ability to scale up antibody product and provide large quantity bulk sizes.

Browse All Primary Antibodies

Recombinant Antibodies Conjugated Primary Antibodies Knockout Validated Antibodies
Biosimilar Antibodies Anti-Idiotype Antibodies Epitope Tag Antibodies

 

Secondary Antibodies & Isotype Controls

Our comprehensive selection of secondary antibodies provides reliable detection and amplification of primary antibody signals. Additionally, our isotype controls offer tools for establishing baseline levels of non-specific binding, ensuring the specificity of your experimental results.

Secondary Antibodies Isotype Controls NorthernLights™ Fluorescent Secondary Antibodies

 

Antibodies by Applications

R&D Systems offers a diverse selection of antibodies with a proven track record of performance. We offer antibodies meticulously validated by our in-house scientists to ensure accurate and reproducible results. Our validated antibody catalog spans various research areas such as immunology, cell biology, and neuroscience, enabling you to discover the ideal antibody for your research needs.

Browse Antibodies by Featured Applications

Flow Cytometry IHC and ICC/IF Blocking/Neutralizing
Western Blot Simple Western Matched Pairs for ELISA
CyTOF-Ready Immunoprecipitation Dual RNAscope™ ISH-IHC

Flow Cytometry Handbook

 

Antibody Quality and Reproducibility

R&D Systems carefully tests every antibody we produce to ensure outstanding performance. Our commitment and approach to quality allows you to be confident in your results and help you generate publication-quality data.

Built for Reproducibility
Results are everything, trust our antibodies. Learn about our commitment to antibody reproducibility.

Customer Guarantee
Learn how our attention to quality allows us to offer a 100% guarantee on all our products and provide first class customer support.

 

Antibody Protocols

Gain insights into the protocols followed by R&D Systems to validate and test our antibodies. Discover the rigorous conditions and methods we utilize to ensure the quality and reliability of our antibodies and reagents.

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Flow Cytometry Protocols IHC and ICC Protocols Western Blot Protocols

 

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Flow Cytometry Training Webinars

In this series of webinars, we will provide investigators with the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment. Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies. In order to maximize the quality of results obtained, researchers should become familiar with the basics when setting up an experiment.

View Flow Cytometry Training Webinars
Flow Cytometry Training Webinar

Additional Resources

 

Featured Content

Featured Content

Immunohistochemistry (IHC) Handbook

Immunohistochemistry (IHC) Handbook

Download our IHC Handbook for techniques, protocols, and troubleshooting tips to help with your IHC experiment.

Download Your Handbook

Featured Content

Custom Antibody Services

Take advantage of R&D Systems in-house antibody production capabilities for your customized antibodies.

Leverage the experience and expertise of our in-house antibody development scientists for your custom project.

Learn About Antibody Custom Services

Featured Content

Cell Marker Tool

Cell Marker Tool

An interactive tool that allows you to explore the cell surface and intracellular markers

Explore Cell Markers

Featured Content

Anti-Mouse Antibodies

Anti-Mouse Antibodies

Enhance your research on Cytokines & Growth Factors with Anti-Mouse Antibodies.

Learn About Anti-Mouse Antibodies

Featured Content

Western Blot Handbook

Western Blot Handbook

Learn about Western Blot systems, protocols, troubleshooting, and more!

Request Your Handbook Today!

Featured Content

GPCR Antibodies

R&D Systems highly validated G Protein-Coupled Receptors (GPCR) Antibodies

Learn about the approach R&D Systems takes to produce the highest performing GPCR antibodies in the industry.

Learn More About Our GPCR Antibodies

Related Information

Antibody Matchmaker
Antibody Production Custom Quote
Custom Antibody Services
Custom Reagents & Contract Services
Immunohistochemistry Troubleshooting Guide