10X NEDD8 Conjugation Reaction Buffer

R&D Systems | Catalog # B-65

R&D Systems
Discontinued Product
B-65 has been discontinued. View all NEDD8 products.

Key Product Details

Species

Multi-Species
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Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: NEDD8

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also known as Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and canine.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Entrez Gene IDs

4738 (Human); 18002 (Mouse); 25490 (Rat)

Gene Symbol

NEDD8

Additional NEDD8 Products

Product Documents for 10X NEDD8 Conjugation Reaction Buffer

Certificate of Analysis

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Product Specific Notices for 10X NEDD8 Conjugation Reaction Buffer

For research use only

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