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5-TAMRA NHS

Catalog #: 7665 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7665/1
5-TAMRA NHS | CAS No. 150810-68-7 | Fluorescent Probes
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Description: Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides

Chemical Name: 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate

Purity: ≥90%

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Biological Activity

5-TAMRA NHS is an orange-fluorescent dye that is commonly used for labeling proteins, peptides and oligonucleotides containing primary amines. Excitation maximum = 546 nm; emission maximum = 580 nm

Please see the protocol for further information on protein/antibody labeling and conjugation.

It is recommended to prepare stock solutions in DMSO.

Optical Data for 5-TAMRA NHS

Emission Color Orange
Brightness
λabs 546 nm
λem 580 nm
Extinction Coefficient (ε) 95,000 M-1cm-1
Quantum Yield (φ) 0.1
Closest Laser line 532 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Alternative For Alexa Fluor® 546

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Technical Data

M.Wt:
527.52
Formula:
C29H25N3O7
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
150810-68-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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