5-TAMRA NHS
Chemical Name: 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
Purity: ≥90%
Biological Activity
Key information: 5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M-1cm-1; A280 = 0.178.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Optical Data for 5-TAMRA NHS
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 546 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| pH Sensitivity | No |
| Correction Factor 280 | 0.178 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor® 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets
Reconstitution Calculator
Molarity Calculator
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for 5-TAMRA NHS
There are currently no reviews for this product. Be the first to review 5-TAMRA NHS and earn rewards!
Have you used 5-TAMRA NHS?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
