Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Cultrex 5X Collagen I Solution
R&D Systems | Catalog # 3457-096-02
Discontinued Product
3457-096-02 has been discontinued.
View all Collagen I products.
Key Product Details
Features
Cultrex 5X Collagen I solution has been developed, manufactured and qualified as a coating for the Cultrex Collagen I Cell Invasion Assays.
Species
Bovine
Product Summary for Cultrex 5X Collagen I Solution
Why Use Cultrex 5X Collagen I Solution?
Cultrex Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of 1 mg/mL.
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Product Specifications
Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Cell Culture Testing
Tested for the ability to support cell attachment and spreading of HT1080 human fibrosarcoma cells.
Performance tested in the Cultrex Collagen I Cell Invasion Assay.
Performance tested in the Cultrex Collagen I Cell Invasion Assay.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Collagen I
Alternate Names
Alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, Collagen 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain
Additional Collagen I Products
Product Documents for Cultrex 5X Collagen I Solution
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
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