Cultrex 5X Collagen IV Solution

Name: Cultrex 5X Collagen IV Solution
Brand: R&D Systems
SKU: 3458-096-02

R&D Systems | Catalog # 3458-096-02

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Product Documents

Discontinued Product

3458-096-02 has been discontinued. View all Collagen IV products.

Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents
Research Areas

Key Product Details

Features

Cultrex 5X Collagen IV solution has been developed, manufactured and qualified as a coating for the Cultrex Collagen IV Cell Invasion Assays.

Species

Mouse

Product Summary for Cultrex 5X Collagen IV Solution

Why Use Cultrex 5X Collagen IV Solution?

Cultrex Mouse Colllagen IV is purified from Engelbreth-Holm-Swarm (EHS) tumor. Collagen IV is a component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.

Product Specifications

Protein Concentration

0.5 mg/mL

Endotoxin Level

≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Cell Culture Testing

Tested for ability to support attachment and spreading of rat PC-12 pheochromocytoma cells.

Performance tested in the Cultrex Collagen IV Cell Invasion Assay.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store in liquid nitrogen for up to 12 months from the date of receipt.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.