Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

R&D Systems | Catalog # S-320

R&D Systems
Discontinued Product
S-320 has been discontinued. View all Proteasome Substrates products.

Key Product Details

Source

Synthetic

Conjugate

7-Amino-4-Methyl Coumarin
View all Proteasome Substrates Small Molecules and Peptides »

Formulation, Preparation, and Storage

S-320
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

For research use only

Related Research Areas

Notch Pathway

Citations for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)

Customer Reviews for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) (2)

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  • Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
    Name: TAKASHI SHIMADA
    Verified Customer | Posted 05/02/2020
    S-320 hydrolysis confirmation by LCMS-SRM S-320: 1 uM i20S: 15 nM PA28a: 150 nM Reaction: 37 °C for 5 h 1:S-320 Ac-ANW-AMC (589.15>414.15) 2:Free AMC (176.25>120.00) 3:S-320 Ac-ANW-COOH (432.20>319.15)
    Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) S-320
  • Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
    Name: TAKASHI SHIMADA
    Verified Customer | Posted 05/02/2020
    Ordinate: fluorescent count, Em 345/Ex 445 Em band path: 9 nm Ex band path: 20 nm Horizontal: time [min] Temp: 37 °C S-320: 5, 25, 50, and 100 uM i20S: 100 ng per assay (100 uL) The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S.
    Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) S-320

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