Bicine

Tocris Bioscience | Catalog # 5881

General purpose buffer
Tocris Bioscience
Discontinued Product
5881 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

General purpose buffer

Product Description

Bicine is a widely used general purpose buffer for biological research. Working pH range in aqueous solution: 7.6-9.0. Used in protein crystallization, ion exchange chromatography and SDS-PAGE.

Product Specifications for Bicine

Molecular Weight

163.17

Formula

C6H13NO4

Storage

Store at RT

Purity

≥99%

Chemical Name

N,N-Bis(2-hydroxyethyl)glycine

CAS Number

150-25-4

PubChem ID

8761

InChI Key

FSVCELGFZIQNCK-UHFFFAOYSA-N

SMILES

OC(CN(CCO)CCO)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 326.34 2000

Preparing Stock Solutions for Bicine

The following data is based on the product molecular weight 163.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
20 mM 0.31 mL 1.53 mL 3.06 mL
100 mM 0.06 mL 0.31 mL 0.61 mL
200 mM 0.03 mL 0.15 mL 0.31 mL
1000 mM 0.01 mL 0.03 mL 0.06 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for Bicine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bicine

For research use only

Customer Reviews for Bicine (1)

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  • Used as a buffer for cell culture: Works well
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/11/2019
    Bicine was used as a buffer for cell culture. Gives publishable result and performed ideally.
    Bicine 5881

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FAQs

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