Caspase-3 Colorimetric Assay Kit

R&D Systems | Catalog # K106-100

R&D Systems
Discontinued Product
K106-100 has been discontinued. View all Caspase-3 products.

Key Product Details

Species

Multi-Species

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Caspase-3

Caspase-3 is a widely expressed dimeric peptidase that is the major executioner caspase, the primary downstream mediator of apoptotic-associated proteolysis. Caspase-3 cleaves multiple substrates including PARP, proIL-16, PKC-gamma and -delta, proCaspases-6, -7, and -9, and beta-Catenin. Normally, it is an inactive, exclusively cytosolic homodimer. During apoptosis, however, proCaspase-3 is activated by cleavage into p20 and p12 subunits, and the p20 subunit is trimmed to yield a p17 subunit. Active Caspase-3 contains two p17 and two p12 subunits.

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Colorimetric Assay Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Caspase-3 Colorimetric Assay Kit

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-3 Colorimetric Assay Kit

Customer Reviews for Caspase-3 Colorimetric Assay Kit (1)

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  • Caspase-3 Colorimetric Assay Kit
    Name: Anonymous
    Verified Customer | Posted 11/28/2021
    Calu-3 human lung cancer cells and A-431 human epidermoid carcinoma cancer cells were treated with Staurosporine (5 μM) for 4 hours or DMSO to induce apoptosis. The cells were then collected, lysed and 150 μg of protein was used to measure Caspase-3 activity at 400 nm, following the instructions of the Caspase-3 Colorimetric Assay Kit (K106-100).
    Caspase-3 Colorimetric Assay Kit K106-100

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