CometAssay 20-well Electrophoresis Starter Kit
CometAssay 20-well Electrophoresis Starter Kit Summary
What is the CometAssay?
The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.
Why Use the CometAssay 20-well Electrophoresis Starter Kit?
CometAssay 20-well Electrophoresisis Starter Kit is designed to eliminate known causes of comet assay variability. It provides an exclusive 20-well CometSlide that is specially treated to promote adherence of low melting point agarose. This eliminates the time consuming and unreliable traditional method of preparing base layers of agarose. The use of 20-well CometSlide High Throughput shortens the overall assay time and allows for the rapid and reliable analysis of large numbers of samples.
• CometAssay Electrophoresis System
• CometAssay Alkaline Control Cells
• CometAssay Lysis Solution
• CometAssay LMAgarose
• 200 mM EDTA, pH 10
• CometSlide (20 Well, 2 Slides)
For research use only. Not for diagnostic use.
Citation for CometAssay 20-well Electrophoresis Starter Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
J Cell Biol, 0;200(2):141-9. 0
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