CometAssay 96-well Electrophoresis Starter Kit
CometAssay 96-well Electrophoresis Starter Kit Summary
What is the CometAssay?
The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.
Why Use the CometAssay 96-well Electrophoresis Starter Kit?
CometAssay 96-well Electrophoresisis Starter Kit is designed to eliminate known causes of comet assay variability and provide a highthroughput method for assessing DNA damage. It provides an exclusive 96-well CometSlide that is specially treated to promote adherence of low melting point agarose. This eliminates the time consuming and unreliable traditional method of preparing base layers of agarose. The use of 96-well CometSlide High Throughput shortens the overall assay time and allows for the rapid and reliable analysis of large numbers of samples.
The complete and standardized system for the comet assay includes a 96 test sample Reagent Kit (1 x 96 well slide), Alkaline Control Cells, and the CometAssay Electrophoresis System II for consistent and reproducible results. Each lot of the Alkaline Control Cells, reagents and CometSlides are tested and qualified for use in the CometAssay ES II.
• CometAssay Lysis Solution
• CometAssay LMAgarose
• 200 mM EDTA, pH 10
• 96-well CometSlide
• CometAssay Alkaline Control Cells
• CometAssay Electrophoresis System II
For research use only. Not for diagnositic use.
Citations for CometAssay 96-well Electrophoresis Starter Kit
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Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction
Authors: E Clementi, L Inglin, E Beebe, C Gsell, Z Garajova, E Markkanen
BMC Biol., 2020;18(1):36. 2020
Norepinephrine-Induced DNA Damage in Ovarian Cancer Cells
Authors: R Lamboy-Car, C Ortiz-Sanc, A Acevedo-Sa, J Matta, A N A Montei, G N Armaiz-P
Int J Mol Sci, 2020;21(6):. 2020
FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
J Cell Biol, 0;200(2):141-9. 0
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