CometAssay Neutral Control Cells
CometAssay Neutral Control Cells SummaryCryopreserved cell preparations contain different levels of DNA damage to be used as controls when performing the comet assay.
• Provides a range of DNA damage for comparison with statistically distinct populations.
• Allows for electrophoresis method to be standardized between individual users.
• Standardized with the CometAssay Electrophoresis System
• Cell populations remain stable in liquid nitrogen for long term storage.
What are CometAssay Neutral Control Cells?
These cryopreserved cell preparations contain different levels of DNA damage to be used as controls when performing the comet assay. Cells are provided at 5 x 10^5 cells/mL. A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).
For research use only. Not for diagnostic use.
Citations for CometAssay Neutral Control Cells
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Recovery from repeated stressors: Physiology and behavior are affected on different timescales in house sparrows
Authors: BMG Gormally, R Estrada, H Yin, LM Romero
Gen. Comp. Endocrinol., 2019-07-19;282(0):113225. 2019-07-19
BRCA1 loss activates cathepsin L-mediated degradation of 53BP1 in breast cancer cells.
Authors: Grotsky D, Gonzalez-Suarez I, Novell A, Neumann M, Yaddanapudi S, Croke M, Martinez-Alonso M, Redwood A, Ortega-Martinez S, Feng Z, Lerma E, Ramon Y Cajal T, Zhang J, Matias-Guiu X, Dusso A, Gonzalo S
J Cell Biol, 2013-01-21;200(2):187-202. 2013-01-21
SIRT1 negatively regulates the activities, functions, and protein levels of hMOF and TIP60.
Authors: Peng L, Ling H, Yuan Z, Fang B, Bloom G, Fukasawa K, Koomen J, Chen J, Lane W, Seto E
Mol Cell Biol, 2012-05-14;32(14):2823-36. 2012-05-14
REG4, NEIL2, and BIRC5 gene expression correlates with gamma-radiation sensitivity in patients with rectal cancer receiving radiotherapy.
Authors: Kobunai T, Watanabe T, Fukusato T
Anticancer Res, 2011-12-01;31(12):4147-53. 2011-12-01
A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest.
Authors: Feng Z, Zhang J
Nucleic Acids Res, 2011-09-27;40(2):726-38. 2011-09-27
What is the purpose of the CometAssay® Control Cells?
The CometAssay Control Cells are designed to help standardize and compare alkaline and neutral electrophoresis methods between individual users and laboratories. The control cells contain known levels of damage.
How should the CometAssay® Control Cells be stored?
The CometAssay Control Cells should be stored in liquid nitrogen. To avoid the accumulation of damage due to freeze thaw cycles, the CometAssay Control Cells should be thawed and frozen in working aliquots. Refer to the CometAssay Control Cells literature for specific instructions.
What cell line is used to generate the CometAssay® Control Cells?
CometAssay Control Cells were prepared using an immortalized human lymphocyte line.
What DNA damaging agent was used to create the CometAssay® Control Cells?
A healthy control cell population (C0) is treated with a DNA damaging agent to increase the amount of damage in populations C1, C2 and C3, respectively. Etoposide is the damaging agent used for Alkaline Control Cells (CC0-CC3) and Bleomycin is the damaging agent used for Neutral Control Cells (NC0-NC3).
Can the Alkaline CometAssay® Control Cells be used for the Neutral CometAssay?
The Alkaline CometAssay Control Cells were qualified for the Alkaline CometAssay while the Neutral CometAssay Control Cells were qualified for the Neutral CometAssay.
Why is it necessary to rinse the CometAssay® Control Cells in PBS before adding to LMAgarose?
The CometAssay Control Cells must be rinsed in PBS to eliminate media and storage factors that reduce adherence of LMAgarose to the slide. PBS without magnesium should be used, as magnesium stimulates nucleases.
Why don't I see a visible cell pellet after centrifugation of the CometAssay® Control Cells?
A pellet may not be visible after centrifugation due to the low number of cells.
Why are there comet tails in the healthy CometAssay® Control Cell population?
The CometAssay Control Cells were prepared from an asynchronous cell population with cells at all stages of the cell cycle. Some percentage of the population will be undergoing DNA replication and may have strand breaks at replication forks.
Improper storage may also lead to DNA damage. Do not store CometAssay Control Cells at -80°C. Store in aliquots in liquid nitrogen.
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