Cultrex 3-D Culture 10X Cell Wash Buffer
Discontinued Product
Cultrex 3-D Culture 10X Cell Wash Buffer Summary
Provides an optimized and standardized solution for washing 3-D cultures in preparation for the isolation of intact 3-D structures from Basement Membrane Extract (BME) Matrix or Laminin I hydrogels as a part of the Cultrex 3-D Culture Cell Harvesting Kit.Why Use Cultrex 3-D Culture 10X Cell Wash Buffer?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture 10X Cell Wash Buffer prepares BME and Laminin I hydrogels for the isolation of 3-D structures.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
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