Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex 3-D Culture 10X Cell Harvesting Buffer
R&D Systems | Catalog # 3448-020-01
Discontinued Product
3448-020-01 has been discontinued.
View all Basement Membrane Extracts products.
Key Product Details
Features
Provides an optimized and standardized solution for the isolation of intact 3-D structures from Basement Membrane Extract (BME) Matrix or Laminin I hydrogels as a part of the Cultrex 3-D Culture Cell Harvesting Kit.
Product Summary for Cultrex 3-D Culture 10X Cell Harvesting Buffer
Why Use Cultrex 3-D Culture 10X Cell Harvesting Buffer?
3-D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels. Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex 3-D Culture 10X Cell Harvesting Buffer depolymerizes BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3-D structures and preparation of cell lysates for subsequent biochemical analysis.
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Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature.
Stability
Product is stable for a minimum of 2 months from date of receipt.
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex 3-D Culture 10X Cell Harvesting Buffer
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
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Application: Cell ProliferationVerified Customer | Posted 04/04/2018
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