Cultrex 5X Collagen I Solution

Catalog # Availability Size / Price Qty
3457-096-02
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Cultrex 5X Collagen I Solution Summary

Cultrex 5X Collagen I solution has been developed, manufactured and qualified as a coating for the Cultrex Collagen I Cell Invasion Assays.

Why Use Cultrex 5X Collagen I Solution?

Cultrex Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of 1 mg/mL.

Specifications

Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Testing Cell Culture
Tested for the ability to support cell attachment and spreading of HT1080 human fibrosarcoma cells.

Performance tested in the Cultrex Collagen I Cell Invasion Assay.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Bovine

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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