Cultrex Human Vitronectin, PathClear

Name: Cultrex Human Vitronectin, PathClear
Brand: R&D Systems
SKU: 3421-001-01
Price: 151 USD

Catalog #: 3421-001-01 Datasheet / COA / SDS

Discontinued Product

3421-001-01 has been discontinued.

View all Vitronectin products.

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Cultrex Human Vitronectin, PathClear Summary

Cultrex Human Vitronectin, Pathclear is a purified stromal extracellular matrix protein that is qualified as a coating solution that promotes for cell attachment to tissue culture vessels.

Key Benefits

• Qualified as a coating substrate that promotes cell attachment
• Support robust, long term proliferation of undifferentiated pluripotent stem cells
• Can be used as a medium additive
• Pathclear-tested to ensure product is free of human virus and pathogens
• Source tissue is LDEV-free

Why Use Cultrex Human Vitronectin?

Cultrex Human Vitronectin, Pathclear is purified from human plasma and is Pathclear certified to be free of common pathogens. Vitronectin is an extracellular, soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa peptide chain with a total molecular weight of 140 kDa. Vitronectin is a major plasma glycoprotein that promotes cellular adhesion and spreading. It also inhibits the membrane damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors. Vitronectin, along with collagen IV, fibronectin, and laminin can support robust, long term proliferation of undifferentiated human embryonic stem cells. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation and differentiation, or as an additive for serum-free media. Vitronectin is purified from human plasma and is 0.2 μm sterile filtered. Cultrex Human Vitronectin is an extracellular matrix coating solution that provides a functionally defined and effective feeder-free surface for the attachment and maintenance of embryonic stem cells in a pluripotent state. It may also be used to supplement customized medium formulations for cell culture.

Specifications

Source

Human plasma

Protein Concentration

1 mg/mL

Endotoxin Level

≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Sterility Testing

No bacterial or fungal growth detected following 14 days in culture

Testing Cell Culture

Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.

Viral Testing

Tested negative by PCR test for mycoplasma and human pathogenic viruses, including: EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, and VZV.

Stability

Product is stable for a minimum of 3 months from date of shipment when stored at ≤ -20 °C. For optimal stability store at ≤ -70 °C. Avoid freeze-thaw cycles.

Shipping Conditions

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Species

Human