Human MMP-2 Quantikine ELISA Kit

R&D Systems | Catalog # DMP2F0

R&D Systems
Discontinued Product
DMP2F0 has been discontinued. View all MMP-2 products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.289 ng/mL

Assay Range

0.78-50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
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Product Summary for Human MMP-2 Quantikine ELISA Kit

The Quantikine Human MMP-2 immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MMP-2 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MMP-2.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Human

Specificity

Natural and recombinant (pro and mature) human MMP-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.30 12.1 18.9 3.96 12.4 19.6
Standard Deviation 0.18 0.65 1.1 0.39 0.86 1.1
CV% 5.5 5.4 5.8 9.8 6.9 5.6

Recovery for Human MMP-2 Quantikine ELISA Kit

The recovery of MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 91-120
Heparin Plasma (n=4) 90 73-105
Serum (n=4) 84 78-89

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MMP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: MMP-2

MMP-2 (matrix metalloproteinase 2), also known as Gelatinase A, is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. The latent proenzyme (proMMP-2) is activated by MT-MMPs, Thrombin, or activated Protein C. Mature MMP-2 activity is inhibited by alpha 2-Macroglobulin or TIMPs 1, 2, 3, and -4. TIMP-2 and -3 can also tether proMMP-2 into cell surface ternary complexes with MT-MMPs. MMP-2 degrades Gelatin (denatured Collagen) and type IV Collagen, the major component of basement membranes. It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. MMP-2 processes and modulates the functions of many other vasoactive and proinflammatory molecules including Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1 beta, S100A8, and S100A9.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Human MMP-2 Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MMP-2 Quantikine ELISA Kit

For research use only

Citations for Human MMP-2 Quantikine ELISA Kit

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