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Human MMP-2 Quantikine ELISA Kit

Catalog #: DMP2F0
16 Citations Datasheet / COA / SDS

Discontinued Product

DMP2F0 has been discontinued.
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Citations (16)
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Summary Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Human MMP-2 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.289 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant (pro and mature) human MMP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human MMP-2 immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MMP-2 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MMP-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.3 12.1 18.9 3.96 12.4 19.6
Standard Deviation 0.18 0.65 1.1 0.39 0.86 1.1
CV% 5.5 5.4 5.8 9.8 6.9 5.6

Recovery

The recovery of MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 91-120
Heparin Plasma (n=4) 90 73-105
Serum (n=4) 84 78-89

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MMP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: MMP-2

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 2
Entrez Gene IDs:
4313 (Human); 17390 (Mouse)
Alternate Names:
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human MMP-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. FFAR2 antagonizes TLR2- and TLR3-induced lung cancer progression via the inhibition of AMPK-TAK1 signaling axis for the activation of NF-?B
    Authors: Kim, MJ;Kim, JY;Shin, JH;Kang, Y;Lee, JS;Son, J;Jeong, SK;Kim, D;Kim, DH;Chun, E;Lee, KY;
    Cell & bioscience
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma
    Authors: AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe
    Oncol Lett, 2020-02-13;19(4):3011-3020.
    Species: Human
    Sample Types: Serum
  3. Relationship between vitreous levels of matrix metalloproteinases and vascular endothelial growth factor in proliferative diabetic retinopathy.
    Authors: Abu El-Asrar, Ahmed M, Mohammad, Ghulam, Nawaz, Mohd Imt, Siddiquei, Mohammad, Van den Eynde, Kathleen, Mousa, Ahmed, De Hertogh, Gert, Opdenakker, Ghislain
    PLoS ONE, 2013-12-31;8(12):e85857.
    Species: Human
    Sample Types: Vitreous Humor
  4. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
    Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
    Autophagy, 2013-12-04;10(2):257-68.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Antiproliferative, antiinvasive, and proapoptotic activity of folate receptor alpha-targeted liposomal doxorubicin in nonfunctional pituitary adenoma cells.
    Authors: Liu X, Ma S, Dai C, Cai F, Yao Y, Yang Y, Feng M, Deng K, Li G, Ma W, Xin B, Lian W, Xiang G, Zhang B, Wang R
    Endocrinology, 2013-03-05;154(4):1414-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Clinical utility of diagnostic guidelines and putative biomarkers in lymphangioleiomyomatosis.
    Respir. Res., 2012-04-18;13(0):34.
    Species: Human
    Sample Types: Serum
  7. Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
    Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
    J Neuroinflammation, 2011-09-09;8(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
    Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
    Oncol Rep, 2011-06-21;26(4):825-31.
    Species: Human
    Sample Types: Serum
  9. Verification of a biomarker discovery approach for detection of Down syndrome in amniotic fluid via multiplex selected reaction monitoring (SRM) assay.
    Authors: Cho CK, Drabovich AP, Batruch I, Diamandis EP
    J Proteomics, 2011-05-20;74(10):2052-9.
    Species: Human
    Sample Types: Amniotic Fluid
  10. Identification of candidate nasopharyngeal carcinoma serum biomarkers by cancer cell secretome and tissue transcriptome analysis: potential usage of cystatin A for predicting nodal stage and poor prognosis.
    Authors: Chang KP, Wu CC, Chen HC
    Proteomics, 2010-07-01;10(14):2644-60.
    Species: Human
    Sample Types: Serum
  11. Effect of arachidonic acid on hypoxia-induced IL-6 production in mouse ES cells: Involvement of MAPKs, NF-kappaB, and HIF-1alpha.
    Authors: Lee SH, Lee YJ, Han HJ
    J. Cell. Physiol., 2010-03-01;222(3):574-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
    Authors: Sesso R, Franco MC
    Am. J. Hypertens., 2009-11-05;23(1):6-11.
    Species: Human
    Sample Types: Plasma
  13. Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia.
    Authors: Karapanagiotidis GT, Antonitsis P, Charokopos N, Foroulis CN, Anastasiadis K, Rouska E, Argiriadou H, Rammos K, Papakonstantinou C
    J Cardiothorac Surg, 2009-11-03;4(0):59.
    Species: Human
    Sample Types: Serum
  14. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009-04-08;76(2):207-14.
    Species: Human
    Sample Types: Urine
  15. 15-Epi-lipoxin A(4) inhibits the progression of endometriosis in a murine model.
    Authors: Chen QH, Zhou WD, Pu DM, Huang QS, Li T, Chen QX
    Fertil. Steril., 2009-03-06;93(5):1440-7.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  16. Significant elevation of plasma matrix metalloproteinase-9 level and its ratio to matrix metalloproteinase-2 in patients with pelvic inflammatory disease.
    Authors: Wang PH, Tsai HT, Tee YT, Lin LY, Yang SF, Hsieh YS
    Fertil. Steril., 2008-10-19;92(5):1679-84.
    Species: Human
    Sample Types: Plasma

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