Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.
NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1435R, filled histogram) or isotype control antibody (Catalog # IC007R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
Shevinsky, L.H. et al. (1982) Cell 30:697.
Kannagi, R. et al. (1983) EMBO J. 2:2355.
Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
Have you used Human/Mouse SSEA-4 Alexa Fluor® 647-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.