Nile Red

Key Product Details

Product Description

Key information: Nile red is a fluorogenic lipid membrane dye.

Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.

Application: fluorescence microscopy.

Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.

Optical Data

Product Specifications for Nile Red

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Nile Red

The following data is based on the product molecular weight 318.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.41 mL	157.05 mL	314.09 mL
0.5 mM	6.28 mL	31.41 mL	62.82 mL
1 mM	3.14 mL	15.70 mL	31.41 mL
5 mM	0.63 mL	3.14 mL	6.28 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Zhanghao High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 2020 PMID: 33208737
• Uribe-Etxebarria Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 2019 PMID: 30549037
• Maes A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 2017 PMID: 28300146

Product Documents for Nile Red