Catalog Number: 2919
Chemical Name: 8-Hydroxypyrene-1,3,6-tris-sulfonic acid-8-1-(2-nitrophenyl)ethyl ether
Biological Activity
Caged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pKa 7.25) upon two-photon excitation (>3000 s-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.
Technical Data
  • M.Wt:
    673.53
  • Formula:
    C24H14NO12Na3S3
  • Solubility:
    Soluble to 100 mM in water and to 100 mM in DMSO
  • Purity:
    >97%
  • Storage:
    Store at -20°C
  • CAS No:
    223759-19-1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones.
    Willoughby et al.
    Pflugers Arch., 1998;436:615
  2. Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate.
    Jasuja et al.
    Biophys.J., 1999;76:1706
  3. The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals.
    Kiskin et al.
    Eur.Biophys.J., 2002;30:588
  4. Neuronal activity regulates diffusion across the neck of dendritic spines.
    Bloodgood and Sabatini
    Science, 2005;310:866
  5. Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration.
    Trigo et al.
    J.Neurosci.Methods, 2009;180:9
  6. Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters.
    Canepari et al.
    J.Neurosci.Methods., 2001;112:29
  7. Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells.
    Soler-Llavina et al.
    Nat.Neurosci., 2006;9:798

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