PIPES, free acid

Tocris Bioscience | Catalog # 5889

Commonly used buffer
Tocris Bioscience
Discontinued Product
5889 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Commonly used buffer
View all Buffers, Chelators and Reagents »

Product Description

Buffer. Working pH range in aqueous solution: 6.1 - 7.5. Recommended for use in solutions containing metal ions. Commonly used in cell culture media, protein crystalization and gel eletrophoresis.

Product Specifications for PIPES, free acid

Molecular Weight

302.40

Formula

C8H18N2O6S2

Storage

Store at RT

Purity

≥98%

Chemical Name

Piperazine-N,N'-bis(2-ethanesulfonic acid)

CAS Number

5625-37-6

PubChem ID

79723

InChI Key

IHPYMWDTONKSCO-UHFFFAOYSA-N

SMILES

O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1M NaOH 151.2 500

Preparing Stock Solutions for PIPES, free acid

The following data is based on the product molecular weight 302.40.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
5 mM 0.66 mL 3.31 mL 6.61 mL
25 mM 0.13 mL 0.66 mL 1.32 mL
50 mM 0.07 mL 0.33 mL 0.66 mL
250 mM 0.01 mL 0.07 mL 0.13 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for PIPES, free acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PIPES, free acid

For research use only

Customer Reviews for PIPES, free acid (1)

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  • PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/13/2019
    Store at 2-8 degrees C
    PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared in 18.2 megohms water and filtered through 0.22-micron filter.PH: 6.8 +/- 0.15 after dilution with DI water.

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