Porcine Luminex® Discovery Assay
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Porcine Luminex® Discovery Assay Summary
|Assay Type||Magnetic bead-based multiplex assay for the Luminex® platform|
|Format||1 x 96-well microplate and Magnetic antibody-coated beads|
|Analytes Detected||Please see analyte list in assay customization tool below.|
|Performance Validation|| Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.
Make your selections below to configure your assay
Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.
If you run into bead region incompatibilities, send your Luminex code to our team and find out more.
How would you like these analytes mixed?
Would you like to split based on dilution?
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.
- Simultaneously profile up to 50 analytes of your choice in one sample
- Choose from a wide menu of analytes, including many unique analytes
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-4 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*
Please allow additional time for international orders.
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Standard/Sample Diluent
- Wash Buffer
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Certificate of Analysis**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
BackgroundPig animal models have an established track record of success in scientific research across a range of study areas, including cardiovascular, clinical work, pharmacology, toxicology, aging, and more recently in stem cell therapies. Porcine models share many similarities to human physiology, anatomy, and mechanisms of human disease, making them a unique valuable experimental model to test new disease therapies, devices and safety and efficacy of new drugs preceding human trials. In addition to transplantation research, a wide range of viral, bacterial, cancer, and inflammatory disease translational research has leveraged the pig’s close homology to humans. At the same time, some “minipig” breeds are providing unique value in longer-term pre-clinical studies due to their ability to age without growing too large, enabling researchers to complete multi-generational studies in comparatively compact timeframes. Emerging human disease research areas for porcine models include the fields of transgenics and regenerative medicine. For the near term, pigs are expected to remain an important translational model to bridge the divide between traditional rodent models and humans in advancing biological experimentation and improving human health.
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Porcine Luminex Discovery Panel Standard Curves
Porcine Luminex Discovery Assay Serum Sample Values Serum samples from 4 porcine were run on the Porcine Luminex Discovery Assay and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.
Is the reconstituted standard a concentrate?
For Discovery Assays, each standard cocktail is a concentrate upon reconstitution with the volume specified on the Certificate of Analysis (CoA). After creating Standard 1 following the table in the product insert, the Standard 1 value is the concentration listed on the CoA.
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