Proteinase K

R&D Systems | Catalog # 4800-30-01

R&D Systems
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Key Product Details

Features

Proteinase K is used for permeabilizing cells and tissues prior to labeling using any TACS In Situ Apoptosis Detection kits.

Key Benefits

  • Can be used with TACS Assay Kits.
  • Remove contaminating/unwanted nucleases or other protein.
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Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Proteinase K

Proteinase K is one of the most active endopeptidases known. It rapidly inactivates endogenous nucleases including RNases and DNases making it suitable for the degradation of proteins during the isolation and manipulation of RNA and DNA. Proteinase K may also be used to permeabilize cells and tissues prior to labeling with in situ Apoptosis Detection Kits. Permeabilization of the sample by Proteinase K creates pores in the cell membrane and facilitates access to the DNA by biotinylated dNTPS.

Alternate Names

Endopeptidase K, PROK, Tritirachium alkaline proteinase

Additional Proteinase K Products

Product Documents for Proteinase K

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Related Research Areas

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