Rat Magnetic Luminex Assay
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Rat Magnetic Luminex Assay Summary
|Assay Type||Magnetic bead-based multiplex assay for the Luminex® platform|
|Format||1 x 96-well microplate and Magnetic antibody-coated beads|
|Analytes Detected||Please see analyte list in assay customization tool below.|
|Performance Validation||Luminex Assays are validated for use with cell culture supernatants, tissue lysates, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.|
Make your selections below to configure your assay
Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.
How would you like these analytes mixed?
Would you like to split based on dilution?
Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.
- Simultaneously profile up to 50 analytes of your choice in one sample
- Choose from a wide menu of analytes, including many unique analytes
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-4 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*
Please allow additional time for international orders.
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Standard/Sample Diluent
- Wash Buffer
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Certificate of Analysis**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Citations for Rat Magnetic Luminex Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
Filter your results:
Comparative effects of dietary n-3 docosapentaenoic acid (DPA), DHA and EPA on plasma lipid parameters, oxidative status and fatty acid tissue composition
Authors: G Drouin, D Catheline, E Guillochea, P Gueret, C Baudry, P Le Ruyet, V Rioux, P Legrand
J. Nutr. Biochem., 2019;63(0):186-196.
Sample Types: Plasma
Cardiovascular toxicity of decabrominated diphenyl ethers (BDE-209) and decabromodiphenyl ethane (DBDPE) in rats
Authors: L Jing, Y Sun, Y Wang, B Liang, T Chen, D Zheng, X Zhao, X Zhou, Z Sun, Z Shi
Sample Types: Serum
Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content
Authors: DK Sung, YS Chang, SI Sung, SY Ahn, WS Park
J Clin Med, 2019;8(4):.
Sample Types: Tissue Homogen
Metformin mediates neuroprotection and attenuates hearing loss in experimental pneumococcal meningitis
Authors: L Muri, ND Le, J Zemp, D Grandgirar, SL Leib
J Neuroinflammation, 2019;16(1):156.
Sample Types: CSF
Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists
Authors: F Evaristo-M, G Sardella-S, TH Kasai-Brun, RMP Campos, P Domizi, MF Santiago, RA de Melo Re, R Mendez-Ote, VT Ribeiro-Re, PM Pimentel-C
Stem Cells Int, 2019;2019(0):7692973.
Sample Types: Cell Culture Supernates
Blunting neuroinflammation with resolvin D1 prevents early pathology in a rat model of Parkinson's disease
Authors: P Krashia, A Cordella, A Nobili, L La Barbera, M Federici, A Leuti, F Campanelli, G Natale, G Marino, V Calabrese, F Vedele, V Ghiglieri, B Picconi, G Di Lazzaro, T Schirinzi, G Sancesario, N Casadei, O Riess, S Bernardini, A Pisani, P Calabresi, MT Viscomi, CN Serhan, V Chiurchiù, M D'Amelio, NB Mercuri
Nat Commun, 2019;10(1):3945.
Sample Types: Plasma
Mycolactone displays anti-inflammatory effects on the nervous system
Authors: C Isaac, A Mauborgne, A Grimaldi, K Ade, M Pohl, C Limatola, Y Boucher, C Demangel, L Guenin-Mac
PLoS Negl Trop Dis, 2017;11(11):e0006058.
Sample Types: Tissue Homogenates
Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury
Authors: GM Bove, MY Harris, H Zhao, MF Barbe
J. Neurol. Sci, 2016;361(0):168-80.
Sample Types: Tissue Homogenates
What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?
R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts.
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