Recombinant Human GST-AMSH-LP Protein, CF

R&D Systems | Catalog # E-551

R&D Systems
Discontinued Product
E-551 has been discontinued. View all AMSH-LP products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q96FJ0

Applications

Bioactivity
View all AMSH-LP Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH-LP protein
Contains an N-terminal GST (glutathione S-transferase) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

79 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial human recombinant GST-AMSH-LP concentration of 100-500 nM when using K63-linked poly-Ubiquitin substrates.  AMSH-LP does not efficiently utilize Ubiquitin-AMC (U-550) as a substrate, though a signal may be obtained when the enzyme is used at a concentration of 500 nM or higher. 

Formulation, Preparation, and Storage

E-551
Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: AMSH-LP

AMSH-like protease (AMSH-LP, also known as STAM-Binding Protein-Like 1, STAMBPL1) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). 436 amino acids in length, AMSH-LP is 56% identical and 73% similar to AMSH, an enzyme that functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. Like AMSH, AMSH-LP only cleaves K63-linked poly-Ubiquitin chains in vitro; however, unlike AMSH, AMSH-LP does not appear to associate with STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.

References

  1. Davies, C.W. et al. (2011) J. Mol. Biol.  413: 416
  2. Sato, Y. et al. (2008) Nature 455: 358

Long Name

STAM-binding Protein-like 1

Alternate Names

ALMalpha, AMSH-FP, AMSHLP, STAMBPL1

Entrez Gene IDs

57559 (Human); 76630 (Mouse); 687696 (Rat)

Gene Symbol

STAMBPL1

UniProt

Q96FJ0

Additional AMSH-LP Products

Product Documents for Recombinant Human GST-AMSH-LP Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GST-AMSH-LP Protein, CF

For research use only

Citations for Recombinant Human GST-AMSH-LP Protein, CF

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