Recombinant Human GST-USP21 Catalytic Domain Protein, CF

R&D Systems | Catalog # E-622

R&D Systems
Discontinued Product
E-622 has been discontinued. View all USP21 products.

Key Product Details

Source

T. ni (baculovirus)

Accession Number

Q9UK80

Applications

Bioactivity
View all USP21 Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human USP21 protein
Contains an N-terminal GST (glutathione S-transferase) tag, Ser196 - Leu565

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

68 kDa

Activity

Recombinant Human GST-USP21 catalytic domain is a Ubiquitin deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP21 concentration of 10-200 nM.

Formulation, Preparation, and Storage

E-622
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP21

USP21 is a deubiquitinating enzyme (DUB) of the C19 peptidase family.  Human USP21 has a predicted molecular weight of 63 kDa and is 96% identical to both mouse and rat orthologues. USP21 has been reported to deubiquitinate histone H2A, a specific tag for epigenetic transcriptional repression.  The transcription factor FOXM1 is also reported to be a substrate for USP21 both in vitro and in vivo, and FOXM1 stabilization by USP21 contributes to cell cycle progression in basal-like breast cancer (BLBC).  USP21 depletion sensitizes BLBC cell lines and mouse xenograft tumors to paclitaxel, a frontline therapy in BLBC treatment, suggesting USP21 inhibitors may be useful in cancer treatment.  This recombinant protein contains an N-terminal GST tag and amino acid residues Ser 196 to Leu 565.

References

  1. Arceci A., et al.  (2019) Cell Rep.  26: 3076
  2. Nakagawa T., et al.  (2008) Genes Dev.  22: 37
  3. Peng L., et al.  (2016) Oncol. Lett.  12: 4531

Long Name

Ubiquitin Specific Protease 21

Alternate Names

ESTM28, USP23, W53272

Entrez Gene IDs

27005 (Human); 30941 (Mouse); 688466 (Rat)

Gene Symbol

USP21

UniProt

Q9UK80

Additional USP21 Products

Product Documents for Recombinant Human GST-USP21 Catalytic Domain Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GST-USP21 Catalytic Domain Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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