Recombinant Human RAP80 UIM Domains Biotin Protein, CF

R&D Systems | Catalog # UBE-235

R&D Systems
Discontinued Product
UBE-235 has been discontinued. View all RAP80 products.

Key Product Details

Species

Human

Applications

Enzyme Activity

Accession Number

NP_057374

Conjugate

Biotin

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in HEPES, NaCl, DTT and Glycerol.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: RAP80

Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-Ub chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked Ub chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with Ub chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetra (or greater) ubiquitin chains. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with other antibodies.

References

  1. Buchberger A. (2002) Trends Cell. Biol. 12 :216-221
  2. Hurley J. H. et al. (2006) Biochem. J. 399 :361-372
  3. Kim H. et al. (2007) Science 316:1202-1205
  4. Kim H. et all. (2008) Mol. Cell. 25:457-61
  5. Sobhian B. et all. (2007) Science 316:1198-1202
  6. Wang B. (2007) Science 316:1194-1198
  7. Wu W. et al. (2008) Cell Div. 31-10
  8. Yan J. et al. (2007) Canc. Res. 15:6647-6656

Long Name

Receptor Associated Protein 80

Alternate Names

RXRIP110, UIMC1, X2HRIP110

Entrez Gene IDs

51720 (Human); 20184 (Mouse); 290997 (Rat)

Gene Symbol

UIMC1

UniProt

NP_057374

Additional RAP80 Products

Product Documents for Recombinant Human RAP80 UIM Domains Biotin Protein, CF

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Product Specific Notices for Recombinant Human RAP80 UIM Domains Biotin Protein, CF

For research use only

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