Recombinant Human SKP1/FBXO7 Complex Protein, CF

Catalog # Availability Size / Price Qty
E3-525-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews

Recombinant Human SKP1/FBXO7 Complex Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/FBX07 Complex protein
Accession #
Predicted Molecular Mass
21 kDa (SKP1), 63 kDa (FBXO7)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-525

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SKP1/FBX07 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases.  FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20 and other mitochondrial proteins.  Evidence from a Drosophila Parkin-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings.  This recombinant protein complex consists of His-tag SKP1 and FLAG-tag FBXO7.

References
  1. Burchell V.S., et al. (2013) Nat. Neurosci.  16: 1257
  2. Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm.  342: 1022
  3. Hsu J.M., et al. (2004) J. Biol. Chem.  279: 32592
  4. Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci.  18: 715
  5. Stott S.R. W., et al. (2019) J. Pathol.  249: 241
Long Name
S-phase Kinase Associated Protein 1/F-Box Only Protein 7
Alternate Names
SKP1/FBX07 Complex

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human SKP1/FBXO7 Complex Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human SKP1/FBXO7 Complex Protein, CF and earn rewards!

Have you used Recombinant Human SKP1/FBXO7 Complex Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review