Recombinant Human SKP1/FBXO7 Complex Protein, CF

Recombinant Human SKP1/FBXO7 Complex Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: SKP1/FBX07 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases.  FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20 and other mitochondrial proteins.  Evidence from a Drosophila Parkin^-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings.  This recombinant protein complex consists of His-tag SKP1 and FLAG-tag FBXO7.

Related Research Areas

• Deubiquitinating Enzymes (DUBs)
• DNA Damage and Repair
• Nucleotide Excision Repair/Transcription Coupled Repair
• Search Ubiquitin-related Enzymes