Human LIF ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91186

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

0.7 pg/mL

Assay Range

3.15 - 200 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human LIF positive serum. No cross-reactivity or interference was detected.

Description

The human LIF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human LIF

精度

Intra-Assay Precision (Precision within an assay) CV% 5.50%

Inter-Assay Precision (Precision between assays) CV% 7.00%

Recovery for Human LIF ELISA Kit (Colorimetric)

Recovery

90%

Scientific Data Images for Human LIF ELISA Kit (Colorimetric)

Human LIF ELISA Kit [NBP1-91186] - Data for Human LIF Platinum ELISA

Kit Contents for Human LIF ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonalantibody to human LIF
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human LIF monoclonal antibody
  • Human LIF Standard, 400 pg/mL
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LIF

Leukemia inhibitory factor (LIF) is a cytokine which was primarily described as a product of activated T lymphocytes and macrophages. It is a variably glycosylated 180 amino acid protein of apparent molecular weight between 35 and 67 kDa depending on its source and glycosylation. The human LIF-gene is located on chromosome 22, the gene product is highly conserved between species. The multivarious nature of LIF is illustrated by its multiple "discoveries" under a variety of synonyms. LIF exerts its pleiotropic functions in a wide variety of systems. Examples for its important and diverse physiological functions are support of growth and inhibition of differentiation of normal embryonic stem cells, stimulation of the production of acute-phase proteins by hepatocytes. A soluble form of LIF can be detected in human body fluids such as serum, whole blood, synovial fluid and urine.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

Additional LIF Products

Product Documents for Human LIF ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human LIF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human LIF ELISA Kit (Colorimetric)

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    • Q: In the product insert it is mentioned that Colored dyes for optional use with this kit are available and will be provided free of charge upon request and for the same it is advised to refer the catalog number NBP2-41404. But the status of the said pack of vials are currently showing as "discontinued". May I request you to kindly confirm whether some other dye would be offered along with this kit and if the customer needs to request for it separately.
      A: For our kit NBP1-91186, we recently changed the format for this ELISA kit. The current format no longer includes the need for the colored dyes that were once included in the kit. We offer these dyes for customers that have purchased this kit in its old formulation and still require these dyes for their analysis. The dyes are no longer required for detection in kits that are purchased moving forward. If it is still intended on performing an ELISA and needs these dyes for their analysis, they can still be provided upon request.
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