Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Complement Factor D/Adipsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin
Ile26-Ser259
Accession # P03953

Specificity

Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Complement Factor D/Adipsin Antibody

Detection of Mouse Complement Factor D/Adipsin antibody by Western Blot.

Detection of Mouse Complement Factor D/Adipsin by Western Blot.

Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse Complement Factor D/Adipsin by Western Blot

Detection of Mouse Complement Factor D/Adipsin by Western Blot

Bone marrow (BM) Adipsin induces bone marrow adiposity expansion during aging.(A) Immunoblot of Adipsin and Adiponectin from plasma of chow-fed male mice at 6, 24, and 76 weeks of age (L.C. = Coomassie staining of the membrane). (B, C) qPCR analyses of gene expression in the BM from tibia (B) and Cfd expression in the epididymal white adipose tissue and subcutaneous white adipose tissue (C) from chow-fed male mice at 26 and 78 weeks of age (n = 5, 5). *p<0.05 for young vs. aging mice. (D–I) Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (D) Representative osmium tetroxide staining and (E) quantification of femoral MAT; (F, G) femoral bone mineral density (BMD) in the cortical (F) and trabecular (G) regions, and (H, I) bone volume normalized by total voume in the cortical (H) and trabecular (I) regions of the femur determined by μCT scans. **p<0.01 for WT vs. Adipsin KO mice. Data represent mean ± SEM. Two-tailed Student’s t-tests were used for statistical analyses.Metabolic phenotyping of Adipsin KO mice during aging.Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (A) Body weight and composition assessed by EchoMRI; (B) insulin tolerance test; (C) GTT; (D) hematoxylin and eosin staining of femoral marrow adipose tissue. (E, F) Average trabecular number (E) and cortical thickness (F) of femurs determined by μCT scans. Data represent mean ± SEM. Two-tailed Student’s t-test was used for statistical analyses. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155972), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Complement Factor D/Adipsin by Western Blot

Detection of Mouse Complement Factor D/Adipsin by Western Blot

Adipsin is robustly induced in the bone marrow (BM) during bone marrow adipose tissue (BMAT) expansion. (D) immunoblot of plasma Adipsin&Adiponectin (APN) – Coomassie staining of the membrane was used as loading control (L.C.) (n = 4, 4);&immunoblot of Adipsin from bone – HSP90 was used as the loading control (n = 4, 4). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155972), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

Adipocyte PPAR gamma acetylation diminishes the metabolic improvements of intermittent fasting (IF) in middle-aged mice. (A–C) Body weight (A), fat mass (B), lean mass (C) of male middle-aged WT and aKQ mice before and after 6 weeks of IF (n = 6, 6). (D) ITT and AUC after fasting for 5 h after 5 weeks of IF. (E) GTT and AUC after an overnight fasting after 6 weeks of IF (n = 6, 6). (F,G) Plasma NEFA and triglyceride (TG) levels after overnight fasting after 6 weeks of IF mice (n = 6, 6). (H,I) WB with quantification of Adipsin and Adiponectin protein levels in plasma and iWAT after 6 weeks of IF (n = 6, 6). (J) Representative H & E staining of BAT, iWAT, and eWAT after IF. Scale bar: 200 µm. (K) qPCR analysis of gene expression levels in BAT (n = 6, 6). Data are presented as mean ± SEM, two-way ANOVA and two-tailed Student’s t-test were used to test significances. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

Adipocyte PPAR gamma acetylation diminishes the metabolic improvements of intermittent fasting (IF) in middle-aged mice. (A–C) Body weight (A), fat mass (B), lean mass (C) of male middle-aged WT and aKQ mice before and after 6 weeks of IF (n = 6, 6). (D) ITT and AUC after fasting for 5 h after 5 weeks of IF. (E) GTT and AUC after an overnight fasting after 6 weeks of IF (n = 6, 6). (F,G) Plasma NEFA and triglyceride (TG) levels after overnight fasting after 6 weeks of IF mice (n = 6, 6). (H,I) WB with quantification of Adipsin and Adiponectin protein levels in plasma and iWAT after 6 weeks of IF (n = 6, 6). (J) Representative H & E staining of BAT, iWAT, and eWAT after IF. Scale bar: 200 µm. (K) qPCR analysis of gene expression levels in BAT (n = 6, 6). Data are presented as mean ± SEM, two-way ANOVA and two-tailed Student’s t-test were used to test significances. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

PPAR gamma acetylation-induced BAT whitening persists despite resveratrol treatment. (A) Body-weight curves of DIO mice co-treated with Rosi and resveratrol (n = 6, 6). (B) ITT and AUC after the co-treatment for 2 weeks (n = 6, 6). (C) GTT and AUC at 3 weeks co-treatment (n = 6, 6). (D). Representative H & E staining of BAT, iWAT, and eWAT after Rosi and resveratrol treatment for 3 weeks. Scale bar: 200 µm. (n = 6, 6). (E) Quantification of WAT adipocyte size (n = 6, 6). (F,G) WB and quantifications in BAT (n = 6, 6). (H,I) Immunohistochemical staining and quantification of UCP1 protein in BAT (n = 3, 3). Data ae presented as mean ± SEM, two-tailed Student’s t-test. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

PPAR gamma acetylation-induced BAT whitening persists despite resveratrol treatment. (A) Body-weight curves of DIO mice co-treated with Rosi and resveratrol (n = 6, 6). (B) ITT and AUC after the co-treatment for 2 weeks (n = 6, 6). (C) GTT and AUC at 3 weeks co-treatment (n = 6, 6). (D). Representative H & E staining of BAT, iWAT, and eWAT after Rosi and resveratrol treatment for 3 weeks. Scale bar: 200 µm. (n = 6, 6). (E) Quantification of WAT adipocyte size (n = 6, 6). (F,G) WB and quantifications in BAT (n = 6, 6). (H,I) Immunohistochemical staining and quantification of UCP1 protein in BAT (n = 3, 3). Data ae presented as mean ± SEM, two-tailed Student’s t-test. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

aKQ mice are sensitive to TZD treatment but with worsened BAT whitening. (A) Body compositions of diet-induced obese (DIO) WT and aKQ mice after a rosiglitazone (Rosi) diet for 7 weeks (n = 13, 9). (B). ITT on Rosi treatment for 0 and 5 weeks (n = 13, 9, 13, and 9). (C) GTT on Rosi treatment for 0 and 6 weeks (n = 13, 9, 13, and 9). (D) AUC of ITT (n = 13, 9, 13, and 9). (E) AUC of GTT (n = 13, 9, 13, and 9). (F,G) WB with quantification of Adipsin and Adiponectin protein levels in plasma (n = 6 and 6). (H) Tissue masses at sacrifice after 7 weeks of Rosi treatment (n = 13, 9). (I,J) WB with quantifications in BAT (n = 6 and 6). * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Complement Factor D/Adipsin by Western Blot

Detection of Complement Factor D/Adipsin by Western Blot

aKQ mice are sensitive to TZD treatment but with worsened BAT whitening. (A) Body compositions of diet-induced obese (DIO) WT and aKQ mice after a rosiglitazone (Rosi) diet for 7 weeks (n = 13, 9). (B). ITT on Rosi treatment for 0 and 5 weeks (n = 13, 9, 13, and 9). (C) GTT on Rosi treatment for 0 and 6 weeks (n = 13, 9, 13, and 9). (D) AUC of ITT (n = 13, 9, 13, and 9). (E) AUC of GTT (n = 13, 9, 13, and 9). (F,G) WB with quantification of Adipsin and Adiponectin protein levels in plasma (n = 6 and 6). (H) Tissue masses at sacrifice after 7 weeks of Rosi treatment (n = 13, 9). (I,J) WB with quantifications in BAT (n = 6 and 6). * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37408258), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Complement Factor D/Adipsin by Western Blot

Detection of Mouse Complement Factor D/Adipsin by Western Blot

CD9 overexpression in the liver ameliorates HFD‐induced hepatic steatosis. AAV‐Ctr and AAV‐CD9 mice were fed on HFD for 12 weeks. A) Schematic diagrams illustrating the experimental design and treatment groups. B) Body weight, liver weight and liver/body weight were determined. C) Liver TG contents were determined enzymatically. D) Representative H&E and Oil Red O staining of livers, and their quantification. E) The mRNA levels of genes related to lipid metabolism in livers. F) Serum levels of liver enzyme activity (ALT and AST) were determined enzymatically. G) Western blot and its quantification of CD9, FLI1 and CFD in liver tissues. Scale bar = 100 µm for C. n = 6 mice per group for B‐G. Data are presented as the mean ± S.E.M. Statistical analysis was performed using t‐test for B‐G. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38837628), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Complement Factor D/Adipsin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Complement Factor D/Adipsin, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Mouse plasma

Reviewed Applications

Read 1 review rated 5 using AF5430 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P03953

Additional Complement Factor D/Adipsin Products

Product Documents for Mouse Complement Factor D/Adipsin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Complement Factor D/Adipsin Antibody

For research use only

Related Research Areas

Complement Pathway

Citations for Mouse Complement Factor D/Adipsin Antibody

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  • Mouse Complement Factor D/Adipsin Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Plasma
    Species: Mouse
    Verified Customer | Posted 08/23/2019
    Mouse Complement Factor D/Adipsin Antibody AF5430

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