Proteome Profiler Human Cytokine Array Kit, Panel A

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines.

 

Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Key Benefits

• Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
• Requires no specialized equipment
• Compatible with LI-COR and chemiluminescence detection

View Data Examples

LI-COR Data Examples
for Human Cytokine Array Panel A

Principle of the Assay

The Proteome Profiler Human Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

• Rectangular 4-Well Multi-dish
• 4 Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
• Array Buffer 4
• Array Buffer 5
• Chemi Reagent 1
• Chemi Reagent 2
• Detection Antibody Cocktail, Human Cytokine Array, Panel A
• Streptavidin-HRP
• Transparency Overlay Template
• Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

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Detection of Multiple Human Cytokines in Cell Culture Supernates. Cells were treated as described below. Following treatment, cell culture supernates were analyzed using the Proteome Profiler Human Cytokine Array Kit, Panel A. Human peripheral blood mononuclear cells were treated with phytohemagglutinin (PHA) for 24 hours. B. TF-1 human erythroleukemia cells were treated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. C. THP-1 human acute monocytic leukemia cells were treated with lipopolysaccharide (LPS) for 16 hours.

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Detection of Multiple Cytokines in Human Serum. Human serum was collected from four donors (A – D), and the relative levels of multiple cytokines were determined using the Proteome Profiler Human Cytokine Array Kit, Panel A.

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Detection of Multiple Cytokines in Human Tissues and Cell Lysates. Extracts were generated from human tissue or cell lines indicated below and analyzed for relative cytokine expression using the Proteome Profiler Human Cytokine Array Kit, Panel A. A. Normal human lung tissue. B. Malignant human lung cancer tissue. C. Human A431 epithelial carcinoma cells. The number on each array image corresponds to one differentially expressed cytokine and is shown in bar graph form in the right panel.