Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Proteome Profiler Human Cytokine Array Kit
R&D Systems | Catalog # ARY005B
Key Product Details
Species
Product Summary for Proteome Profiler Human Cytokine Array Kit
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.
Key Benefits
- Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 5
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
|---|---|---|
| C5a | IL-4 | IL-27 |
| CD40 Ligand | IL-5 | IL-32 alpha |
| G-CSF | IL-6 | CXCL10/IP-10 |
| GM-CSF | IL-8 | CXCL11/I-TAC |
| CXCL1/GRO alpha | IL-10 | CCL2/MCP-1 |
| CCL1/I-309 | IL-12 p70 | MIF |
| ICAM-1 | IL-13 | MIP-1 alpha/MIP-1 beta |
| IFN-gamma | IL-16 | CCL5/RANTES |
| IL-1 alpha | IL-17 | CXCL12/SDF-1 |
| IL-1 beta | IL-17E | Serpin E1/PAI-1 |
| IL-1ra | IL-18 | TNF-alpha |
| IL-2 | IL-21 | TREM-1 |
Assays for Analytes represented in the Human Cytokine Array Kit
| Analyte | Quantikine® ELISA Kits | Quantikine® HS (High Sensitivity) ELISA Kits | QuantiGlo® ELISA Kits | DuoSet® ELISA Development Systems |
|---|---|---|---|---|
| C5a | DY2037 | |||
| CD40 Ligand | DCDL40 | DY617 | ||
| G-CSF | DCS50 | HSTCS0 | DY214 | |
| GM-CSF | DGM00 | HSGM0 | DY215 | |
| CXCL1/GRO alpha | DGR00 | DY275 | ||
| CCL1/I-309 | DY272 | |||
| ICAM-1 | DCIM00 | DY720 | ||
| IFN-gamma | DIF50C | DY285B | ||
| IL-1 alpha | DLA50 | DY200 | ||
| IL-1 beta | DLB50 | HSLB00C | QLB00B | DY201 |
| IL-1ra | DRA00B | DY280 | ||
| IL-2 | D2050 | Q2000B | DY202 | |
| IL-4 | D4050 | HS400 | DY204 | |
| IL-5 | D5000B | DY205 | ||
| IL-6 | D6050B | HS600B | Q6000B | DY206 |
| CXCL8/IL-8 | D8000C | HS800 | Q8000B | DY208 |
| IL-10 | D1000B | HS100C | Q1000 | DY217B |
| IL-12 p70 | D1200 | HS120 | DY1270 | |
| IL-13 | D1300B | DY213 | ||
| IL-16 | D1600 | DY316 | ||
| IL-17 | D1700 | DY317 | ||
| IL-17E | ||||
| IL-18 | 7620 | |||
| IL-21 | ||||
| IL-27 | ||||
| IL-32 alpha | ||||
| CXCL10/IP-10 | DIP100 | DY266 | ||
| CXCL11/I-TAC | DCX110 | DY672 | ||
| CCL2/MCP-1 | DCP00 | DY279 | ||
| MIF | DMF00B | DY289 | ||
| CCL3/MIP-1 alpha | DMA00 | DY270 | ||
| CCL4/MIP-1 beta | DMB00 | DY271 | ||
| Serpin E1/PAI-1 | DSE100, DTSE100 | |||
| CCL5/RANTES | DRN00B | DY278 | ||
| CXCL12/SDF-1 | DSA00 | DY350 | ||
| TNF-alpha | DTA00C | HSTA00D | QTA00B | DY210 |
| TREM-1 | DTRM10B | DY1278B |
Scientific Data Images for Proteome Profiler Human Cytokine Array Kit
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (500 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure).
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates.
The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure).
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples.
200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.1. ICAM-1/CD54
2. MIF
3. Complment Component C5/C5a
4. CXCL1/GRO alpha
5. IL-8
6. IL-16
7. IL-18
8. CCL5/RANTES
9. IL-1ra/IL-1F3
10. Serpin E1/PAI-1
Formulation, Preparation, and Storage
Shipping
Storage
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Human Cytokine Array Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Human Cytokine Array Kit
For research use only
Citations for Proteome Profiler Human Cytokine Array Kit
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Verified Customer | Posted 08/20/2023the provided protocol is straightforward.
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Verified Customer | Posted 09/29/2021Excellent results
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Verified Customer | Posted 01/25/2017Tested condition media of MSCs from the umbilical cord co-cultured with NK cells. The goal of this experiments was to detect chages in inflammatory factors in the system when MSCs were cultured with NK cells. The lack of detected secreted proteins is promising because this suggests that MSCs do not up-regulate large inflammatory proteins following immune cell activation. Left image : MSC cultured in RPMI media (Control) Right image: MSC co-cultured with NK cells in same RPMI media
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FAQs for Proteome Profiler Human Cytokine Array Kit
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Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.