Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.

Key Benefits

  • Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
 

 

Principle of the Assay

The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 5
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
C5a IL-4 IL-27
CD40 Ligand IL-5 IL-32 alpha
G-CSF IL-6 CXCL10/IP-10
GM-CSF IL-8 CXCL11/I-TAC
CXCL1/GRO alpha IL-10 CCL2/MCP-1
CCL1/I-309 IL-12 p70 MIF
ICAM-1 IL-13 MIP-1 alpha/MIP-1 beta
IFN-gamma IL-16 CCL5/RANTES
IL-1 alpha IL-17 CXCL12/SDF-1
IL-1 beta IL-17E Serpin E1/PAI-1
IL-1ra IL-18 TNF-alpha
IL-2 IL-21 TREM-1

Assays for Analytes represented in the Human Cytokine Array Kit

Analyte Quantikine® ELISA Kits Quantikine® HS (High Sensitivity) ELISA Kits QuantiGlo® ELISA Kits DuoSet® ELISA Development Systems
C5a       DY2037
CD40 Ligand DCDL40     DY617
G-CSF DCS50 HSTCS0   DY214
GM-CSF DGM00 HSGM0   DY215
CXCL1/GRO alpha DGR00     DY275
CCL1/I-309       DY272
ICAM-1 DCIM00     DY720
IFN-gamma DIF50     DY285
IL-1 alpha DLA50     DY200
IL-1 beta DLB50 HSLB00C QLB00B DY201
IL-1ra DRA00B     DY280
IL-2 D2050   Q2000B DY202
IL-4 D4050 HS400   DY204
IL-5 D5000B     DY205
IL-6 D6050 HS600B Q6000B DY206
CXCL8/IL-8 D8000C HS800 Q8000B DY208
IL-10 D1000B HS100C Q1000 DY217B
IL-12 p70 D1200 HS120   DY1270
IL-13 D1300B     DY213
IL-16 D1600     DY316
IL-17 D1700     DY317
IL-17E        
IL-18 7620      
IL-21        
IL-27      

DY2526

IL-32 alpha        
CXCL10/IP-10 DIP100     DY266
CXCL11/I-TAC DCX110     DY672
CCL2/MCP-1 DCP00     DY279
MIF DMF00B     DY289
CCL3/MIP-1 alpha DMA00     DY270
CCL4/MIP-1 beta DMB00     DY271
Serpin E1/PAI-1 DSE100, DTSE100    

DY1786

CCL5/RANTES DRN00B     DY278
CXCL12/SDF-1 DSA00     DY350
TNF-alpha DTA00C HSTA00D QTA00B DY210
TREM-1 DTRM10B     DY1278B
 

 

Data Examples
PROFILING PROTEINS IN CELL CULTURE SUPERNATES
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PROFILING PROTEINS IN CELL LYSATES

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (50 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure).

PROFILING PROTEINS IN CELL LYSATES
View Larger Image

PROFILING PROTEINS IN CELL LYSATES

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates. The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure).

PROFILING PROTEINS IN TISSUE LYSATES
View Larger Image

PROFILING PROTEINS IN TISSUE LYSATES

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples. 200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

  1. ICAM-1/CD54
  2. MIF
  3. Complment Component C5/C5a
  4. CXCL1/GROα
  5. IL-8
  6. IL-16
  7. IL-18
  8. CCL5/RANTES
  9. IL-1ra/IL-1F3
  10. Serpin E1/PAI-1
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

116 Citations: Showing 1 - 10
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Species
Sample Type
  1. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
    Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
    Autophagy, 2017;0(0):0. 2017
  2. miRNA-1246 induces pro-inflammatory responses in mesenchymal stem/stromal cells by regulating PKA and PP2A
    Authors: A Bott, N Erdem, S Lerrer, A Hotz-Wagen, C Breunig, K Abnaof, A Wörner, H Wilhelm, E Münsterman, A Ben-Baruch, S Wiemann
    Oncotarget, 2017;8(27):43897-43914. 2017
  3. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
    Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
    Int J Mol Sci, 2017;18(2):. 2017
  4. Orai and TRPC channel characterization in Fc?RI-mediated calcium signaling and mediator secretion in human mast cells
    Authors: HE Wajdner, J Farrington, C Barnard, PT Peachell, CG Schnackenb, JP Marino, X Xu, K Affleck, M Begg, EP Seward
    Physiol Rep, 2017;5(5):. 2017
  5. Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling
    Authors: HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim
    PLoS ONE, 2017;12(4):e0175159. 2017
  6. Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells
    Authors: EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee
    Oncotarget, 2017;8(1):1438-1448. 2017
  7. Peri-foci adipose-derived stem cells promote chemoresistance in breast cancer
    Authors: WL Yeh, CF Tsai, DR Chen
    Stem Cell Res Ther, 2017;8(1):177. 2017
  8. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017;0(0):. 2017
  9. Cancer-Associated Fibroblasts Share Characteristics and Pro-tumorigenic Activity with Mesenchymal Stromal Cells
    Authors: L Borriello, R Nakata, MA Sheard, GE Fernandez, R Sposto, J Malvar, L Blavier, H Shimada, S Asgharzade, RC Seeger, YA DeClerck
    Cancer Res., 2017;0(0):. 2017
  10. Downregulation of UHRF1 increases tumor malignancy by activating the CXCR4/AKT-JNK/IL-6/Snail signaling axis in hepatocellular carcinoma cells
    Authors: JH Kim, JW Shim, DY Eum, SD Kim, SH Choi, K Yang, K Heo, MT Park
    Sci Rep, 2017;7(1):2798. 2017
  11. NOX2-dependent ATM kinase activation dictates pro-inflammatory macrophage phenotype and improves effectiveness to radiation therapy
    Authors: Q Wu, A Allouch, A Paoletti, C Leteur, C Mirjolet, I Martins, L Voisin, F Law, H Dakhli, E Mintet, M Thoreau, Z Muradova, M Gauthier, O Caron, F Milliat, DM Ojcius, F Rosselli, E Solary, N Modjtahedi, E Deutsch, JL Perfettini
    Cell Death Differ., 2017;0(0):. 2017
  12. The Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal Endothelial Cells
    Authors: A Hongo, N Okumura, M Nakahara, EP Kay, N Koizumi
    Invest. Ophthalmol. Vis. Sci., 2017;58(9):3325-3334. 2017
  13. Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis
    Authors: Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng
    Stem Cells, 2017;0(0):. 2017
  14. Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
    Proc Natl Acad Sci USA, 2016;0(0):. 2016
  15. Serum Cytokines in Young Pediatric Patients with Congenital Cardiac Shunts and Altered Pulmonary Hemodynamics
    Mediators Inflamm, 2016;2016(0):7672048. 2016
  16. Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists
    Sci Rep, 2016;6(0):34145. 2016
  17. Soluble alpha-enolase activates monocytes by CD14-dependent TLR4 signalling pathway and exhibits a dual function
    Authors: C Guillou, M Fréret, E Fondard, C Derambure, G Avenel, ML Golinski, M Verdet, O Boyer, F Caillot, P Musette, T Lequerré, O Vittecoq
    Sci Rep, 2016;6(0):23796. 2016
  18. The MNK-1/eIF4E pathway as a new therapeutic pathway to target inflammation and remodelling in asthma
    Cell Signal, 2016;28(10):1555-1562. 2016
  19. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
    Authors: Matthew N Svalina
    Sci Rep, 2016;6(0):27012. 2016
  20. The oncometabolite R-2-hydroxyglutarate activates NF-?B-dependent tumor-promoting stromal niche for acute myeloid leukemia cells
    Sci Rep, 2016;6(0):32428. 2016
  21. NF-?B-HOTAIR axis links DNA damage response, chemoresistance and cellular senescence in ovarian cancer
    Authors: AR Öze?, DF Miller, ON Öze?, F Fang, Y Liu, D Matei, T Huang, KP Nephew
    Oncogene, 2016;0(0):. 2016
  22. Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer
    Authors: ME Legrier, I BiŠche, J Gaston, A Beurdeley, V Yvonnet, O D‚as, A Thuleau, S Chƒteau-Jo, JL Servely, S Vacher, M Lassalle, S Depil, GC Tucker, JJ Fontaine, MF Poupon, S Roman-Roma, JG Judde, D Decaudin, S Cairo, E Marangoni
    Br. J. Cancer, 2016;114(2):177-87. 2016
  23. XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer.
    Authors: Morton J, Bird G, Keysar S, Astling D, Lyons T, Anderson R, Glogowska M, Estes P, Eagles J, Le P, Gan G, McGettigan B, Fernandez P, Padilla-Just N, Varella-Garcia M, Song J, Bowles D, Schedin P, Tan A, Roop D, Wang X, Refaeli Y, Jimeno A
    Oncogene, 2016;35(3):290-300. 2016
  24. Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
    Authors: Skolekova S, Matuskova M, Bohac M, Toro L, Demkova L, Gursky J, Kucerova L
    Cell Commun Signal, 2016;14(1):4. 2016
  25. Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents
    Authors: J Gaston, L Cheradame, V Yvonnet, O Deas, MF Poupon, JG Judde, S Cairo, V Goffin
    Oncotarget, 2016;7(47):77205-77224. 2016
  26. MicroRNAs regulate cytokine responses in gingival epithelial cells
    Infect Immun, 2016;0(0):. 2016
  27. MEK and TAK1 regulate apoptosis in colon cancer cells with KRAS-dependent activation of proinflammatory signaling
    Mol Cancer Res, 2016;0(0):. 2016
  28. Fibrocytes Differ from Macrophages but Can Be Infected with HIV-1.
    Authors: Hashimoto M, Nasser H, Bhuyan F, Kuse N, Satou Y, Harada S, Yoshimura K, Sakuragi J, Monde K, Maeda Y, Welbourn S, Strebel K, Abd El-Wahab E, Miyazaki M, Hattori S, Chutiwitoonchai N, Hiyoshi M, Oka S, Takiguchi M, Suzu S
    J Immunol, 2015;195(9):4341-50. 2015
  29. Exosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts.
    Authors: Paggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim Y, Adam J, Lichter P, Solary E, Berchem G, Moussay E
    Blood, 2015;126(9):1106-17. 2015
  30. Cytokine profiling of docetaxel-resistant castration-resistant prostate cancer.
    Authors: Mahon K, Lin H, Castillo L, Lee B, Lee-Ng M, Chatfield M, Chiam K, Breit S, Brown D, Molloy M, Marx G, Pavlakis N, Boyer M, Stockler M, Daly R, Henshall S, Horvath L
    Br J Cancer, 2015;112(8):1340-8. 2015
  31. The role of TLR8 signaling in acute myeloid leukemia differentiation.
    Authors: Ignatz-Hoover, J J, Wang, H, Moreton, S A, Chakrabarti, A, Agarwal, M K, Sun, K, Gupta, K, Wald, D N
    Leukemia, 2015;29(4):918-26. 2015
  32. Diurnal rhythms of serum and plasma cytokine profiles in healthy elderly individuals assessed using membrane based multiplexed immunoassay.
    Authors: Altara R, Manca M, Hermans K, Daskalopoulos E, Brunner-La Rocca H, Hermans R, Struijker-Boudier H, Blankesteijn M
    J Transl Med, 2015;13(0):129. 2015
  33. In vitro Study of a Novel Stent Coating Using Modified CD39 Messenger RNA to Potentially Reduce Stent Angioplasty-Associated Complications.
    Authors: Abraham M, Nolte A, Reus R, Behring A, Zengerle D, Avci-Adali M, Hohmann J, Peter K, Schlensak C, Wendel H, Krajewski S
    PLoS ONE, 2015;10(9):e0138375. 2015
  34. New aspects of RpoE in uropathogenic Proteus mirabilis.
    Authors: Liu M, Kuo K, Chien H, Tsai Y, Liaw S
    Infect Immun, 2015;83(3):966-77. 2015
  35. IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
    Authors: Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
    Oncogene, 2015;34(7):815-25. 2015
  36. Regulation of prostate stromal fibroblasts by the PIM1 protein kinase.
    Authors: Zemskova M, Song J, Cen B, Cerda-Infante J, Montecinos V, Kraft A
    Cell Signal, 2015;27(1):135-46. 2015
  37. Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells.
    Authors: Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D
    J Immunol, 2015;194(6):2578-86. 2015
  38. Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
    Authors: Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A
    PLoS ONE, 2015;10(3):e0122065. 2015
  39. Moraxella catarrhalis adhesin UspA1-derived recombinant fragment rD-7 induces monocyte differentiation to CD14+CD206+ phenotype.
    Authors: Xie, Qi, Brackenbury, Louise S, Hill, Darryl J, Williams, Neil A, Qu, Xun, Virji, Mumtaz
    PLoS ONE, 2014;9(3):e90999. 2014
  40. The biological effect of large single doses: a possible role for non-targeted effects in cell inactivation.
    Authors: Veldwijk M, Zhang B, Wenz F, Herskind C
    PLoS ONE, 2014;9(1):e84991. 2014
  41. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
    Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
    Placenta, 2014;35(12):1049-56. 2014
  42. Human astrocytes: secretome profiles of cytokines and chemokines.
    Authors: Choi S, Lee H, Lim I, Satoh J, Kim S
    PLoS ONE, 2014;9(4):e92325. 2014
  43. Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
    Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
    PLoS ONE, 2014;9(7):e102239. 2014
  44. Differential MSC activation leads to distinct mononuclear leukocyte binding mechanisms.
    Authors: Kota, Daniel J, DiCarlo, Bryan, Hetz, Robert A, Smith, Philippa, Cox, Charles, Olson, Scott D
    Sci Rep, 2014;4(0):4565. 2014
  45. DeltaNp63 promotes pediatric neuroblastoma and osteosarcoma by regulating tumor angiogenesis.
    Authors: Bid, Hemant K, Roberts, Ryan D, Cam, Maren, Cam M, Audino A, Audino, Anthony, Kurmasheva, Raushan, Lin, Jiayuh, Houghton, Peter J, Cam, Hakan, Cam H
    Cancer Res, 2014;74(1):320-9. 2014
  46. Human adipose-derived stromal/stem cells induce functional CD4+CD25+FoxP3+CD127- regulatory T cells under low oxygen culture conditions.
    Authors: Frazier T, McLachlan J, Gimble J, Tucker H, Rowan B
    Stem Cells Dev, 2014;23(9):968-77. 2014
  47. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63. 2014
  48. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014;5(0):4715. 2014
  49. Slit2-Robo4 pathway modulates lipopolysaccharide-induced endothelial inflammation and its expression is dysregulated during endotoxemia.
    Authors: Zhao H, Anand A, Ganju R
    J Immunol, 2014;192(1):385-93. 2014
  50. A cannabigerol derivative suppresses immune responses and protects mice from experimental autoimmune encephalomyelitis.
    Authors: Carrillo-Salinas F, Navarrete C, Mecha M, Feliu A, Collado J, Cantarero I, Bellido M, Munoz E, Guaza C
    PLoS ONE, 2014;9(4):e94733. 2014
  51. The RNA chaperone Hfq is involved in stress tolerance and virulence in uropathogenic Proteus mirabilis.
    Authors: Wang M, Chien H, Tsai Y, Liu M, Liaw S
    PLoS ONE, 2014;9(1):e85626. 2014
  52. Interleukin-6 induces the lineage commitment of bone marrow-derived mesenchymal multipotent cells through down-regulation of Sox2 by osteogenic transcription factors.
    Authors: Yoon D, Kim Y, Lee S, Lee K, Park K, Jang Y, Lee J
    FASEB J, 2014;28(7):3273-86. 2014
  53. Cytokine/chemokine secretion and proteomic identification of upregulated annexin A1 from peripheral blood mononuclear cells cocultured with the liver fluke Opisthorchis viverrini.
    Authors: Hongsrichan N, Intuyod K, Pinlaor P, Khoontawad J, Yongvanit P, Wongkham C, Roytrakul S, Pinlaor S
    Infect Immun, 2014;82(5):2135-47. 2014
  54. Development, characterization, and reversal of acquired resistance to the MEK1 inhibitor selumetinib (AZD6244) in an in vivo model of childhood astrocytoma.
    Authors: Bid H, Kibler A, Phelps D, Manap S, Xiao L, Lin J, Capper D, Oswald D, Geier B, DeWire M, Smith P, Kurmasheva R, Mo X, Fernandez S, Houghton P
    Clin Cancer Res, 2013;19(24):6716-29. 2013
  55. miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate the invasion and tumorigenic and angiogenic responses of a cancer cell line to microtubule-targeting drugs.
    Authors: Kim Y, Park D, Kim H, Choi M, Lee H, Lee Y, Choe J, Kim Y, Jeoung D
    J Biol Chem, 2013;288(51):36502-18. 2013
  56. Cold atmospheric plasma (CAP) changes gene expression of key molecules of the wound healing machinery and improves wound healing in vitro and in vivo.
    Authors: Arndt S, Unger P, Wacker E, Shimizu T, Heinlin J, Li Y, Thomas H, Morfill G, Zimmermann J, Bosserhoff A, Karrer S
    PLoS ONE, 2013;8(11):e79325. 2013
  57. SMRT-GPS2 corepressor pathway dysregulation coincides with obesity-linked adipocyte inflammation.
    Authors: Toubal A, Clement K, Fan R, Ancel P, Pelloux V, Rouault C, Veyrie N, Hartemann A, Treuter E, Venteclef N
    J Clin Invest, 2013;123(1):362-79. 2013
  58. Leukemia cells induce changes in human bone marrow stromal cells.
    Authors: Civini S, Jin P, Ren J, Sabatino M, Castiello L, Jin J, Wang H, Zhao Y, Marincola F, Stroncek D
    J Transl Med, 2013;11(0):298. 2013
  59. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis.
    Authors: Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L
    J Clin Invest, 2013;123(1):189-205. 2013
  60. Increased circulation of galectin-3 in cancer induces secretion of metastasis-promoting cytokines from blood vascular endothelium.
    Authors: Chen C, Duckworth C, Zhao Q, Pritchard D, Rhodes J, Yu L
    Clin Cancer Res, 2013;19(7):1693-704. 2013
  61. Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.
    Authors: Kakkola L, Denisova O, Tynell J, Viiliainen J, Ysenbaert T, Matos R, Nagaraj A, Ohman T, Kuivanen S, Paavilainen H, Feng L, Yadav B, Julkunen I, Vapalahti O, Hukkanen V, Stenman J, Aittokallio T, Verschuren E, Ojala P, Nyman T, Saelens X, Dzeyk K, Kainov D
    Cell Death Dis, 2013;4(0):e742. 2013
  62. Gremlin-1 is an inhibitor of macrophage migration inhibitory factor and attenuates atherosclerotic plaque growth in ApoE-/- Mice.
    Authors: Muller I, Schonberger T, Schneider M, Borst O, Ziegler M, Seizer P, Leder C, Muller K, Lang M, Appenzeller F, Lunov O, Buchele B, Fahrleitner M, Olbrich M, Langer H, Geisler T, Lang F, Chatterjee M, de Boer J, Tietge U, Bernhagen J, Simmet T, Gawaz M
    J Biol Chem, 2013;288(44):31635-45. 2013
  63. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
    Acta Pharmacol. Sin., 2012;33(9):1204-16. 2012
  64. Primary tumor hypoxia recruits CD11b+/Ly6Cmed/Ly6G+ immune suppressor cells and compromises NK cell cytotoxicity in the premetastatic niche.
    Authors: Sceneay J, Chow M, Chen A, Halse H, Wong C, Andrews D, Sloan E, Parker B, Bowtell D, Smyth M, Moller A
    Cancer Res, 2012;72(16):3906-11. 2012
  65. Comparison of endometrial regenerative cells and bone marrow stromal cells.
    Authors: Wang, Huan, Jin, Ping, Sabatino, Marianna, Ren, Jiaqiang, Civini, Sara, Bogin, Vladimir, Ichim, Thomas E, Stroncek, David F
    J Transl Med, 2012;10(0):207. 2012
  66. Obatoclax, saliphenylhalamide, and gemcitabine inhibit influenza a virus infection.
    Authors: Denisova, Oxana V, Kakkola, Laura, Feng, Lin, Stenman, Jakob, Nagaraj, Ashwini, Lampe, Johanna, Yadav, Bhagwan, Aittokallio, Tero, Kaukinen, Pasi, Ahola, Tero, Kuivanen, Suvi, Vapalahti, Olli, Kantele, Anu, Tynell, Janne, Julkunen, Ilkka, Kallio-Kokko, Hannimar, Paavilainen, Henrik, Hukkanen, Veijo, Elliott, Richard, De Brabander, Jef K, Saelens, Xavier, Kainov, Denis E
    J Biol Chem, 2012;287(42):35324-32. 2012
  67. Differential interaction of the two related fungal species Candida albicans and Candida dubliniensis with human neutrophils.
    J. Immunol., 2012;189(5):2502-11. 2012
  68. Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts.
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Other Experimental DetailsTested condition media of MSCs from the umbilical cord co-cultured with NK cells. The goal of this experiments was to detect chages in inflammatory factors in the system when MSCs were cultured with NK cells. The lack of detected secreted proteins is promising because this suggests that MSCs do not up-regulate large inflammatory proteins following immune cell activation. Left image : MSC cultured in RPMI media (Control)Right image: MSC co-cultured with NK cells in same RPMI media

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