Human G-CSF Quantikine ELISA Kit

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PDCS50
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G-CSF ELISA Kit ELISA
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Citations (31)
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Human G-CSF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
20 pg/mL
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human G-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human G-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure G-CSF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human G-CSF and antibodies raised against the protein. It has been shown to accurately quantitate recombinant human G-CSF. Results obtained using natural human G-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human G-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 177 515 1047 143 818 1514
Standard Deviation 4.5 9.4 22 17 72 77.1
CV% 2.5 1.8 2.1 11.9 8.8 5.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 280 827 1696 176 1094 2169
Standard Deviation 7.8 14.1 19.5 7.3 34.6 81.6
CV% 2.8 1.7 1.1 4.1 3.2 3.8

Recovery

The recovery of G-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 94 85-110
Citrate Plasma (n=5) 90 78-111
EDTA Plasma (n=5) 93 80-117
Heparin Plasma (n=5) 87 76-103
Serum (n=5) 88 75-106

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of G-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human G-CSF ELISA Linearity

Data Examples

Human G-CSF ELISA Cell Culture Supernate Standard Curve

Human G-CSF ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Long Name:
Granulocyte Colony Stimulating Factor
Entrez Gene IDs:
1440 (Human); 12985 (Mouse)
Alternate Names:
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human G-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
    Authors: PH Jaïs, E Decroly, E Jacquet, M Le Boulch, A Jaïs, O Jean-Jean, H Eaton, P Ponien, F Verdier, B Canard, S Goncalves, S Chiron, M Le Gall, P Mayeux, M Shmulevitz
    Nucleic Acids Res., 2019;0(0):.
    Species: Transgenic Hamster
    Sample Types: Cell Culture Supernates
  2. Stroma-derived IL-6, G-CSF and Activin-A mediated dedifferentiation of lung carcinoma cells into cancer stem cells
    Authors: CFD Rodrigues, E Serrano, MI Patrício, MM Val, P Albuquerqu, J Fonseca, CMF Gomes, AJ Abrunhosa, A Paiva, L Carvalho, MF Botelho, L Almeida, IM Carreira, MC Alpoim
    Sci Rep, 2018;8(1):11573.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer
    Authors: C Thålin, S Lundström, C Seignez, M Daleskog, A Lundström, P Henriksson, T Helleday, M Phillipson, H Wallén, M Demers
    PLoS ONE, 2018;13(1):e0191231.
    Species: Human
    Sample Types: Plasma
  4. Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps
    Authors: NA Johdi, L Mazlan, I Sagap, R Jamal
    Cytokine, 2017;99(0):35-42.
    Species: Human
    Sample Types: Serum
  5. Peptidoglycan accelerates granulopoiesis through a TLR2- and MyD88-dependent pathway
    Authors: M Takehara, S Seike, T Takagishi, K Kobayashi, M Nagahama
    Biochem. Biophys. Res. Commun., 2017;487(2):419-425.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study
    Authors: O Freynet, J Marchal-So, F Jean-Louis, A Mailleux, B Crestani, P Soler, L Michel
    Respir Res, 2016;17(1):36.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of ?-tubulin deactylase
    Nucleic Acids Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Multimodal Regulation of NET Formation in Pregnancy: Progesterone Antagonizes the Pro-NETotic Effect of Estrogen and G-CSF
    Authors: Stavros Giaglis
    Front Immunol, 2016;7(0):565.
    Species: Human
    Sample Types: Serum
  9. Regulation of Circulating Hematopoietic Stem/Progenitor Cells in Preterm Infants with Septicemia
    Stem Cells Dev, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  10. Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
    Authors: Günay Kalender
    Ther Clin Risk Manag, 2016;12(0):1841-1847.
    Species: Human
    Sample Types: Plasma
  11. LPS-Induced G-CSF Expression in Macrophages Is Mediated by ERK2, but Not ERK1.
    Authors: Chang S, Lin S, Yang H, Chou Y, Gao J, Lu S
    PLoS ONE, 2015;10(6):e0129685.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Granulocyte colony-stimulating factor receptor signalling via Janus kinase 2/signal transducer and activator of transcription 3 in ovarian cancer.
    Authors: Kumar, J, Fraser, F W, Riley, C, Ahmed, N, McCulloch, D R, Ward, A C
    Br J Cancer, 2014;110(1):133-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
    Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
    Oncol Lett, 2014;8(3):1112-1118.
    Species: Human
    Sample Types: Serum
  14. Novel immunocompetent murine tumor model for evaluation of conditionally replication-competent (oncolytic) murine adenoviral vectors.
    Authors: Robinson M, Li B, Ge Y, Ko D, Yendluri S, Harding T, VanRoey M, Spindler KR, Jooss K
    J. Virol., 2009;83(8):3450-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
    Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
    Cytokine, 2009;47(3):206-13.
    Species: Human
    Sample Types: Plasma
  16. Randomized trial and pharmacokinetic study of pegfilgrastim versus filgrastim after dose-intensive chemotherapy in young adults and children with sarcomas.
    Authors: Fox E, Widemann BC, Hawkins DS, Jayaprakash N, Dagher R, Aikin AA, Bernstein D, Long L, Mackall C, Helman L, Steinberg SM, Balis FM
    Clin. Cancer Res., 2009;15(23):7361-7.
    Species: Human
    Sample Types: Serum
  17. Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs.
    Authors: Makam M, Diaz D, Laval J, Gernez Y, Conrad CK, Dunn CE, Davies ZA, Moss RB, Herzenberg LA, Herzenberg LA, Tirouvanziam R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5779-83.
    Species: Human
    Sample Types: Plasma
  18. Hematopoietic progenitor cell mobilization and harvesting in children with malignancies: do the advantages of pegfilgrastim really translate into clinical benefit?
    Authors: Merlin E, Zohar S, Jérôme C, Veyrat-Masson R, Marceau G, Paillard C, Auvrignon A, Le Moine P, Gandemer V, Sapin V, Halle P, Boiret-Dupre N, Chevret S, Demeocq F, Dubray C, Kanold J
    Bone Marrow Transplant., 2009;43(12):919-25.
    Species: Human
    Sample Types: Serum
  19. Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.
    Authors: Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL
    J. Immunol., 2008;180(5):3520-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
    Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
    Allergy, 2008;63(10):1359-67.
    Species: Human
    Sample Types: Nasal Lavage
  22. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
    Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
    Crit. Care Med., 2008;36(9):2583-9.
    Species: Human
    Sample Types: Plasma
  23. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
    Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
    Blood, 2005;105(2):830-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Is granulocyte colony-stimulating factor level predictive for human IVF outcome?
    Authors: Salmassi A, Schmutzler AG, Schaefer S, Koch K, Hedderich J, Jonat W, Mettler L
    Hum. Reprod., 2005;20(9):2434-40.
    Species: Human
    Sample Types: Follicular Fluid
  26. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
    Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
    Exp. Hematol., 2004;32(5):441-9.
    Species: Human
    Sample Types: Plasma
  27. Spontaneous circulation of myeloid-lymphoid-initiating cells and SCID-repopulating cells in sickle cell crisis.
    Authors: Lamming CE, Augustin L, Blackstad M, Lund TC, Hebbel RP, Verfaillie CM
    J. Clin. Invest., 2003;111(6):811-9.
    Species: Human
    Sample Types: Serum
  28. Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation.
    Authors: de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D
    Br. J. Haematol., 2002;118(3):875-84.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
  29. Distinct hematopoietic support by two human stromal cell lines.
    Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
    Exp. Hematol., 2001;29(6):736-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Glioblastoma causing granulocytosis by secretion of granulocyte-colony-stimulating factor.
    Authors: Hintzen RQ, Voormolen J, Sonneveld P, van Duinen SG
    Neurology, 2000;54(1):259-61.
    Species: Human
    Sample Types: CSF
  31. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
    Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
    J Clin Invest, 1998;101(4):890-8.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human G-CSF Quantikine ELISA kit is used for Diagnostic use.