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Proteome Profiler Mouse Phospho-RTK Array Kit

Catalog #: ARY014
37 Citations 8 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 39 different RTK antibodies
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ARY014
The Mouse Phospho-RTK Array detects multiple tyrosine phosphorylated receptors in untreated and ligand-treated cell lysates.
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Citations (37)
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Proteome Profiler Mouse Phospho-RTK Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of mouse receptor tyrosine kinase phosphorylation. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

  • Detects phosphorylation of 39 mouse receptors simultaneously
  • Requires no specialized equipment

Principle of the Assay

The Proteome Profiler Mouse Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Tyrosine phosphorylation of the captured proteins is detected with an HRP-conjugated pan phospho-tyrosine antibody (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount phosphorylation in the bound analyte.

Why Use an Antibody Array to Detect Receptor Phosphorylation?

Determining the phosphorylation of multiple receptors in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple phosphorylations in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Anti-Phospho-Tyrosine-HRP Detection Antibody
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 
Simultaneously detect the relative phosphorylation of these RTKs in a single sample
EGF R PDGF R alpha VEGF R3
ErbB2 PDGF R beta MuSK
ErbB3 SCF R EphA1
ErbB4 Flt-3 EphA2
FGF R2 (IIIc) M-CSF R EphA3
FGF R3 c-Ret EphA6
FGF R4 Tie-1 EphA7
Insulin R Tie-2 EphA8
IGF-I R TrkA EphB1
Axl TrkB EphB2
DtK TrkC EphB4
Mer VEGF R1 EphB6
HGF R VEGF R2  
MSP R    

 

Assays for analytes represented in the Mouse Phospho-Receptor Tyrosine Kinase Array Kit

  DuoSet® ELISA Development Reagents DuoSet® IC ELISA Development Reagents (Total) DuoSet® IC ELISA Development Reagents (Phospho) Quantikine® ELISA Kits Cell-based ELISA Kits
EGF R          
ErbB2          
ErbB3          
ErbB4          
FGF R2 (IIIc)          
FGF R3          
FGF R4          
Insulin R          
IGF-I R          
Axl DY854        
DtK DY759        
Mer DY591        
HGF R DY527        
MSP R          
PDGF R alpha          
PDGF R beta          
SCF R          
Flt-3          
M-CSF R          
c-Ret          
Tie-1          
Tie-2     DYC2816    
TrkA          
TrkB          
TrkC          
VEGF R1 DY471     MVR100  
VEGF R2 DY1558B     MVR200B  
VEGF R3 DY743        
MuSK          
EphA1          
EphA2          
EphA3          
EphA6          
EphA7          
EphA8          
EphB1          
EphB2          
EphB4          
EphB6          

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

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Product Datasheet
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Scientific Data

N/A The Mouse Phospho-RTK Array detects multiple tyrosine phosphorylated receptors in untreated and ligand-treated cell lysates. View Larger

Detection of Multiple Tyrosine Phosphorylated Receptors in Cell Lysates by the Mouse Phospho-RTK Array. The amount of lysate incubated with each array is indicated in the figure. Data shown are from 2 minute (Panels A, B, and C) or 5 minute (Panel D) exposure to X-ray film.A. M1 mouse myeloid leukemia cells were either untreated or treated with 500 ng/mL recombinant mouse Flt-3 Ligand (Catalog # 427-FL) for 5 minutes.B. NMuMG mouse mammary gland epithelial cells were either untreated or treated with 200 ng/mL recombinant mouse EGF (Catalog # 2028-EG) for 5 minutes.C. Hepa 1-6 mouse hepatoma cells were either untreated or treated with 1 µg/mL recombinant human insulin (Sigma, Catalog # I9278).D. HEK293 human embryonic kidney cells transfected with mouse EphA1 were either untreated or treated with 3 µg/mL mouse Ephrin-A1 (Catalog # 602-A1) and 0.3 µg/mL goat anti-human IgG Fc (Catalog # G-102-C) for 20 minutes.

Citations for Proteome Profiler Mouse Phospho-RTK Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Osteoclast-derived extracellular vesicles are implicated in sensory neurons sprouting through the activation of epidermal growth factor signaling
    Authors: E Neto, L Leitão, JC Mateus, DM Sousa, CJ Alves, M Aroso, AC Monteiro, F Conceição, ROC Oreffo, J West, P Aguiar, M Lamghari
    Cell & bioscience, 2022-08-14;12(1):127.  2022-08-14
  2. MAP3K4 promotes fetal and placental growth by controlling the receptor tyrosine kinases IGF1R/IR and Akt signaling pathway�
    Authors: CH Perry, NA Mullins, RBA Sweileh, NAM Shendy, PA Roberto, AL Broadhurst, HA Nelson, GA Miranda-Ca, AN Abell
    The Journal of Biological Chemistry, 2022-07-31;0(0):102310.  2022-07-31
  3. EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli
    Authors: S Song, Z Yu, Y You, C Liu, X Xie, H Lv, F Xiao, Q Zhu, C Qin
    Cell Death & Disease, 2022-04-15;13(4):351.  2022-04-15
  4. Loss of Tpl2 activates compensatory signaling and resistance to EGFR/MET dual inhibition in v-RAS transduced keratinocytes
    Authors: MB Kelley, TJ Geddes, M Ochiai, NM Lampl, WW Kothmann, SR Fierstein, V Kent, K DeCicco-Sk
    PLoS ONE, 2022-03-24;17(3):e0266017.  2022-03-24
  5. Impact of silk hydrogel secondary structure on hydrogel formation, silk leaching and in vitro response
    Authors: G Egan, S Phuagkhaop, SAL Matthew, P Connolly, FP Seib
    Scientific Reports, 2022-03-08;12(1):3729.  2022-03-08
  6. CCN2-induced lymphangiogenesis is mediated by the integrin alphavbeta5-ERK pathway and regulated by DUSP6
    Authors: S Hashiguchi, T Tanaka, R Mano, S Kondo, S Kodama
    Scientific Reports, 2022-01-18;12(1):926.  2022-01-18
  7. Transcriptional profiling of mESC-derived tendon and fibrocartilage cell fate switch
    Authors: DA Kaji, AM Montero, R Patel, AH Huang
    Nature Communications, 2021-07-09;12(1):4208.  2021-07-09
  8. Cell-Autonomous Role of EGFR in Spontaneous Duodenal Tumors in LRIG1 Null Mice
    Authors: H Niitsu, Y Lu, WJ Huh, AM Love, JL Franklin, RJ Coffey
    Cellular and Molecular Gastroenterology and Hepatology, 2021-05-11;0(0):.  2021-05-11
  9. The HSP-RTK-Akt axis mediates acquired resistance to Ganetespib in HER2-positive breast cancer
    Authors: CE Eyermann, JD Haley, EM Alexandrov
    Cell Death & Disease, 2021-01-26;12(1):126.  2021-01-26
  10. An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1
    Authors: B Lee, H Shin, JE Oh, J Park, M Park, SC Yang, JH Jun, SH Hong, H Song, HJ Lim
    Autophagy, 2020-06-17;0(0):1-18.  2020-06-17
  11. TAS-115 inhibits PDGFR&alpha/AXL/FLT-3 signaling and suppresses lung metastasis of osteosarcoma
    Authors: N Yasuda, S Takenaka, S Nakai, T Nakai, S Yamada, Y Imura, H Outani, K Hamada, H Yoshikawa, N Naka
    FEBS Open Bio, 2020-03-30;0(0):.  2020-03-30
  12. Combined MEK and PI3K/p110b inhibition as a novel targeted therapy for malignant mesothelioma displaying sarcomatoid features
    Authors: M Marqués, R Tranchant, B Risa-Ebrí, ML Suárez-Sol, LC Fernández, E Carrillo-d, N Del Pozo, J Martínez d, C Meiller, Y Allory, Y Blum, C Pirker, B Hegedus, ST Barry, A Carnero, W Berger, D Jean, FX Real
    Cancer Res., 2020-01-07;0(0):.  2020-01-07
  13. PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment
    Authors: J Naipauer, S Rosario, S Gupta, C Premer, O Méndez-Sol, M Schlesinge, V Ponzinibbi, V Jain, L Gay, R Renne, HL Chan, L Morey, D Salyakina, M Abba, S Williams, JM Hare, PJ Goldschmid, EA Mesri
    PLoS Pathog., 2019-12-27;15(12):e1008221.  2019-12-27
  14. Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons
    Authors: N Haq, C Schmidt-Hi, FJ Sialana, L Ciani, JP Heller, M Stewart, L Bentley, S Wells, RJ Rodenburg, PM Nolan, E Forsythe, MC Wu, G Lubec, P Salinas, M Häusser, PL Beales, S Christou-S
    PLoS Biol., 2019-09-03;17(9):e3000414.  2019-09-03
  15. Norgestrel, a Progesterone Analogue, Promotes Significant Long-Term Neuroprotection of Cone Photoreceptors in a Mouse Model of Retinal Disease
    Authors: SL Roche, O Kutsyr, N Cuenca, TG Cotter
    Invest. Ophthalmol. Vis. Sci., 2019-07-01;60(8):3221-3235.  2019-07-01
  16. EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke
    Authors: AS Ernst, LI Böhler, AM Hagenston, A Hoffmann, S Heiland, C Sticht, M Bendszus, M Hecker, H Bading, HH Marti, T Korff, R Kunze
    Acta Neuropathol Commun, 2019-02-05;7(1):15.  2019-02-05
  17. KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis
    Authors: LE Cavallin, Q Ma, J Naipauer, S Gupta, M Kurian, P Locatelli, P Romanelli, M Nadji, PJ Goldschmid, EA Mesri
    PLoS Pathog., 2018-07-09;14(7):e1007175.  2018-07-09
  18. Heat shock factor 1 confers resistance to lapatinib in ERBB2-positive breast cancer cells
    Authors: A Yallowitz, A Ghaleb, L Garcia, EM Alexandrov, N Marchenko
    Cell Death Dis, 2018-05-24;9(6):621.  2018-05-24
  19. Glycosylation controls cooperative PECAM-VEGFR2-?3 integrin functions at the endothelial surface for tumor angiogenesis
    Authors: R Imamaki, K Ogawa, Y Kizuka, Y Komi, S Kojima, N Kotani, K Honke, T Honda, N Taniguchi, S Kitazume
    Oncogene, 2018-05-02;0(0):.  2018-05-02
  20. Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro
    Authors: D Zhang, B Shen, Y Zhang, N Ni, Y Wang, F Xianqun, H Sun, P Gu
    J. Cell. Mol. Med., 2017-09-18;0(0):.  2017-09-18
  21. Gemcitabine-induced TIMP1 attenuates therapy response and promotes tumor growth and liver metastasis in pancreatic cancer
    Authors: Z D'Costa, K Jones, A Azad, R van Stipho, SY Lim, AL Gomes, P Kinchesh, SC Smart, WG McKenna, FM Buffa, OJ Sansom, RJ Muschel, E O'Neill, E Fokas
    Cancer Res., 2017-08-01;0(0):.  2017-08-01
  22. Axonal outgrowth, neuropeptides expression and receptors tyrosine kinase phosphorylation in 3D organotypic cultures of adult dorsal root ganglia
    Authors: E Neto, CJ Alves, L Leitão, DM Sousa, IS Alencastre, F Conceição, M Lamghari
    PLoS ONE, 2017-07-24;12(7):e0181612.  2017-07-24
  23. HBEGF promotes gliomagenesis in the context of Ink4a/Arf and Pten loss
    Authors: CH Shin, JP Robinson, JA Sonnen, AE Welker, DX Yu, MW VanBrockli, SL Holmen
    Oncogene, 2017-04-03;0(0):.  2017-04-03
  24. Effective combinatorial immunotherapy for castration-resistant prostate cancer
    Authors: X Lu, JW Horner, E Paul, X Shang, P Troncoso, P Deng, S Jiang, Q Chang, DJ Spring, P Sharma, JA Zebala, DY Maeda, YA Wang, RA DePinho
    Nature, 2017-03-20;543(7647):728-732.  2017-03-20
  25. Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling
    Authors: AK Piper, SE Ross, GM Redpath, FA Lemckert, N Woolger, A Bournazos, PA Greer, RB Sutton, ST Cooper
    Cell. Signal, 2017-02-10;33(0):30-40.  2017-02-10
  26. Insulin-mediated signaling facilitates resistance to PDGFR inhibition in proneural hPDGFB-driven gliomas
    Authors: DA Almiron Bo, C Ran, MC Havrda, H Liu, Y Hitoshi, Z Zhang, C Cheng, M Ung, MA Israel
    Mol. Cancer Ther, 2017-01-30;0(0):.  2017-01-30
  27. OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency
    Authors: M Ullrich, M Weber, AM Post, S Popp, J Grein, M Zechner, H Guerrero G, A Kreis, AG Schmitt, N Üçeyler, KP Lesch, K Schuh
    Mol. Psychiatry, 2017-01-10;0(0):.  2017-01-10
  28. Epidermal growth factor receptor inhibition reduces angiogenesis via hypoxia-inducible factor-1alpha and Notch1 in head neck squamous cell carcinoma.
    Authors: Wang W, Zhao Z, Ma S, Yu G, Liu B, Zhang L, Zhang W, Kulkarni A, Sun Z, Zhao Y
    PLoS ONE, 2015-02-27;10(2):e0119723.  2015-02-27
  29. HPV16-associated tumors control myeloid cell homeostasis in lymphoid organs, generating a suppressor environment for T cells.
    Authors: Stone S, Rossetti R, Bolpetti A, Boccardo E, Souza P, Lepique A
    J Leukoc Biol, 2014-06-26;96(4):619-31.  2014-06-26
  30. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
    Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
    J Clin Invest, 2014-04-24;124(6):2696-708.  2014-04-24
  31. Lack of Matrilin-2 favors liver tumor development via Erk1/2 and GSK-3beta pathways in vivo.
    Authors: Fullar A, Baghy K, Deak F, Peterfia B, Zsak Y, Tatrai P, Schaff Z, Dudas J, Kiss I, Kovalszky I
    PLoS ONE, 2014-04-01;9(4):e93469.  2014-04-01
  32. Decorin deficiency promotes hepatic carcinogenesis.
    Authors: Horvath Z, Kovalszky I, Fullar A, Kiss K, Schaff Z, Iozzo R, Baghy K
    Matrix Biol, 2013-12-18;35(0):194-205.  2013-12-18
  33. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals.
    Authors: Miyabayashi K, Ijichi H, Mohri D, Tada M, Yamamoto K, Asaoka Y, Ikenoue T, Tateishi K, Nakai Y, Isayama H, Morishita Y, Omata M, Moses H, Koike K
    Cancer Res, 2013-02-01;73(7):2221-34.  2013-02-01
  34. Estrogenic compounds are not always cardioprotective and can be lethal in males with genetic heart disease.
    Authors: Haines, Christop, Harvey, Pamela A, Luczak, Elizabet, Barthel, Kristen, Konhilas, John P, Watson, Peter A, Stauffer, Brian L, Leinwand, Leslie A
    Endocrinology, 2012-07-09;153(9):4470-9.  2012-07-09
  35. Lentiviral vector induced insertional haploinsufficiency of Ebf1 causes murine leukemia.
    Authors: Heckl D, Schwarzer A, Haemmerle R, Steinemann D, Rudolph C, Skawran B, Knoess S, Krause J, Li Z, Schlegelberger B, Baum C, Modlich U
    Mol. Ther., 2012-04-03;20(6):1187-95.  2012-04-03
  36. Deletion of the epidermal growth factor receptor in renal proximal tubule epithelial cells delays recovery from acute kidney injury.
    Authors: Chen J, Chen JK, Harris RC
    Kidney Int., 2012-03-14;82(1):45-52.  2012-03-14
  37. Specific glycosaminoglycans modulate neural specification of mouse embryonic stem cells.
    Authors: Pickford CE, Holley RJ, Rushton G, Stavridis MP, Ward CM, Merry CL
    Stem Cells, 2011-04-01;29(4):629-40.  2011-04-01

FAQs

  1. What are the phosphorylation site(s) of the 49 different tyrosine residues on the RTKs?

    • This kit uses a pan anti-phospho-tyrosine antibody as the detection antibody, which means it is capable of detecting phosphorylation at any available tyrosine. It is not designed to be specific for one phosphorylation site on each molecule. For instance, PDGF R alpha has multiple sites of tyrosine phosphorylation (see the “PTM/Processing” section of http://www.uniprot.org/uniprot/P16234 for a list of the currently identified sites). 

  2. Can the Mouse Phospho-RTK Array be used for measuring the relative levels of total RTKs present in a sample?

    • No. Although the array kit uses capture antibodies that recognize both phosphorylated and unphosphorylated RTKs, the detection antibody is a pan anti-phospho-tyrosine antibody which only detects phosphorylated tyrosines on activated RTKs.

  3. To confirm positive signal for an RTK by IP-Western blot, are the capture antibodies from the Mouse Phospho-RTK Array Kit offered separately?

    • The identities of the capture antibodies on the Mouse Phospho-RTK Array are considered proprietary. However, to help investigators confirm their array results, Bio-Techne offers antibodies for each RTK detectable in the array kit. These antibodies are validated for Western blot and can be found in our catalog. A pan anti-phospho-tyrosine-HRP detection antibody is also available (Catalog # HAM1676).

View all Proteome Profiler Antibody Array FAQs

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Reviews for Proteome Profiler Mouse Phospho-RTK Array Kit

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Proteome Profiler Mouse Phospho-RTK Array Kit
By Anonymous on 06/09/2023

Use of RTK array to assay FACS sorted cells from murine lungs.

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Felipe Rodrigues on 10/16/2022

Sample from FACS sorted cells, so limited amount of protein but still able to see some phospho RTK. Would definitely use again.

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Anonymous on 04/20/2019

Array works and produces publishable results. Not happy with the pho-PDGFR antibody, sinse it appear to cross react with something in mouse tumor lysate

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Anonymous on 03/27/2019
Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Anonymous on 04/16/2018
Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Mia Huang on 07/05/2017

Lysates from mouse embryonic stem cells applied to membranes using instructions provided. Western blots were clean and worked great!

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Natasha Tracey on 05/16/2017

Worked really well on mouse tumour lysates.

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
Proteome Profiler Mouse Phospho-RTK Array Kit
By Natalia Marchenko on 06/29/2016

Mammary tumors extracts from MMTV-Neu mice with different p53 genotypes (p53wt vs mutp53)

Proteome Profiler Mouse Phospho-RTK Array Kit ARY014