VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.
Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
R&D Systems | Catalog # MVR100
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)
Sensitivity
15.2 pg/mL
Assay Range
125-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse sVEGF R1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 265 | 1383 | 2174 | 250 | 1339 | 2139 |
| Standard Deviation | 19 | 55 | 69 | 21 | 96 | 134 |
| CV% | 7.2 | 4.0 | 3.2 | 8.4 | 7.2 | 6.3 |
Recovery for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 96 | 82-110 |
| EDTA Plasma (n=6) | 106 | 92-120 |
| Serum (n=6) | 106 | 96-119 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
Mouse VEGF R1/Flt-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGFR1/Flt-1
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
Additional VEGFR1/Flt-1 Products
Product Documents for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
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Customer Reviews for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit (2)
3 out of 5
2 Customer Ratings
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Sample Tested: mouse serumVerified Customer | Posted 09/05/2025The suggested sample dilution of 1:2 may not be accurate for all study models. Users should test a range of 1:2-1:10 to have a precise result. Otherwise, it may involve a significant extrapolation, leading to not-so-accurate numbers. Thanks.
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Sample Tested: Heparin PlasmaVerified Customer | Posted 01/16/2024The kit detects endogenous VEGFR1 but not mouse recombinant VEGFR1 made by R & D systems
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. We investigated and the lab testing confirmed that our MVR100 kit DOES detect 471-F1
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Protocols
View specific protocols for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit (MVR100):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
