Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

Name: Mouse VEGFR2/Flk-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MVR200B
Price: 680 USD
Availability: InStock

R&D Systems | Catalog # MVR200B

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Product Documents

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

0.049 ng/mL

Assay Range

0.16-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)

View all VEGFR2/KDR/Flk-1 ELISA Kits »

Product Summary for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse sVEGF R2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	24	24	24
Mean (ng/mL)	0.63	1.40	3.83	0.57	1.28	3.50
Standard Deviation	0.04	0.07	0.21	0.06	0.10	0.21
CV%	6.3	5.0	5.5	10.5	7.8	6.0

Recovery for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

The recovery of mouse sVEGF R2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	105	100-111
Plasma (n=4)	108	89-116
Serum (n=3)	104	82-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sVEGF R2 in each matrix were diluted with Calibrator Diluent and then assayed.

Scientific Data Images for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

Mouse VEGF R2/KDR/Flk-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR2/KDR/Flk-1

VEGFR2/KDR/Flk-1 (vascular endothelial growth factor receptor 2) is a transmembrane receptor tyrosine kinase that mediates the angiogenic effects of VEGF-A and VEGF-C. It is expressed primarily on vascular endothelial cells and endothelial cell progenitors. It is also expressed on endometrial epithelium, hematopoietic stem cells, liver sinusoidal endothelial cells, Sertoli cells and Leydig cells, platelets and megakaryocytes, sensory and autonomic neurons, Schwann cells, Muller glial cells, retinal progenitors, and osteoblasts. VEGFR2 can associate with VEGFR1, and a soluble form of VEGFR2 can circulate in the serum.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

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Certificate of Analysis

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Product Specific Notices for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.