SAHA

Tocris Bioscience | Catalog # 4652

Class I and II HDAC inhibitor
Tocris Bioscience

Key Product Details

Description

Class I and II HDAC inhibitor

Alternative Names

Vorinostat
View all Class I Histone Deacetylases Inhibitors »

Product Description

SAHA inhibits Class I and II histone deacetylases (HDACs); induces accumulation of acetylated histones H2A, H2B, H3 and H4 in transformed cultured cells. Suppresses cell growth in a range of cancer cell lines; induces apoptosis in cutaneous T cell lymphoma cells in vitro. Activates autophagy. SAHA increases efficiency of transcription factor-induced reprogramming of mouse embryonic fibroblasts (MEF) to induced pluripotent stem cells (iPSC). Enhances adeno-associated virus transduction of HeLa cells. Also induces activation and replication of latent HIV in vitro.

Product Specifications for SAHA

Molecular Weight

264.32

Formula

C14H20N2O3

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

N-Hydroxy-N'-phenyloctanediamide

CAS Number

149647-78-9

PubChem ID

5311

InChI Key

WAEXFXRVDQXREF-UHFFFAOYSA-N

SMILES

O=C(CCCCCCC(NO)=O)NC1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 26.43 100

Preparing Stock Solutions for SAHA

The following data is based on the product molecular weight 264.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.78 mL 18.92 mL 37.83 mL
5 mM 0.76 mL 3.78 mL 7.57 mL
10 mM 0.38 mL 1.89 mL 3.78 mL
50 mM 0.08 mL 0.38 mL 0.76 mL

Calculators

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Product Documents for SAHA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SAHA

For research use only

Customer Reviews for SAHA (1)

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  • Excellent histone deacetylase inhibitor
    Name: Luiz Alexandre Magno
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: PC12
    Verified Customer | Posted 11/25/2021
    SAHA can be easily resuspended in DMSO. Make a stock solution with a concentration that will not exceed 0.01% of DMSO in the cell culture to avoid toxicity.
    Our lab uses this drug to monitor transcriptional activity of genes that depend on class I, II and IV of histone deacetylase. In the experiment shown in the figure, we have treated PC12 cells with 3 different concentrations of SAHA for 3h. We found increased transcriptional activity of PI3K gene upon SAHA treatment in a dose-dependent manner.
    SAHA 4652

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