Western Blot Imaging Systems
All FluorChem Imaging systems are discontinued as of October 2021. Service for these instruments will continue for FluorChem E, M, and R systems through October 15th, 2026. If you have any questions or concerns, please contact Technical Support or email instrument-support@bio-techne.com.
Assistance with product applications & usage, and technical support for instruments & service contracts.
| Catalog # | Description |
| 60-12596-00 | ChemiGlow Chemiluminescence Substrate Kit |
| WB201 | MultiFluor Western Blotting Kit (2-color, option 1) Red (Goat Anti-Rabbit) and Green (Goat Anti-Mouse) |
| WB202 | MultiFluor Western Blotting Kit (2-color, option 2) Red (Goat Anti-Mouse) and Green (Goat Anti-Rabbit) |
| WB301 | MultiFluor Western Blotting Kit (3-color, option 1) Red (Goat Anti-Rabbit), Green (Goat Anti-Mouse) and Blue (Donkey Anti-Chicken) |
| WB302 | MultiFluor Western Blotting Kit (3-color, option 2) Red (Goat Anti-Mouse), Green (Goat Anti-Rabbit) and Blue (Donkey Anti-Chicken) |
| Catalog # | Description |
| 041-058 | MultiFluor Green Secondary (Goat-Anti-Rabbit IgG) |
| 041-059 | MultiFluor Red Secondary (Goat-Anti-Mouse IgG) |
| 041-061 | MultiFluor Red Secondary (Goat-Anti-Rabbit IgG) |
| 041-063 | MultiFluor Green Secondary (Goat-Anti-Mouse IgG) |
| 041-064 | MultiFluor Blue Secondary (Donkey-Anti-Chicken IgG) |
| 041-050 | MultiFluor Blocking Solution |
| 041-052 | MultiFluor Antibody Wash |
| 041-054 | MultiFluor Final Wash |
| P-150H | Thermal Paper - High Contrast |
FluorChem Imaging Systems Accessories
| Catalog # | Description |
| 91-13840-00 | Green Emission Filter |
| HB-500 | Blue Emission Filter |
| EBR-500K | Orange Emission Filter |
| SYPRO-500 | Red Emission Filter |
| 75-14083-00 | Replacement UV Bulb (365 nm) |
| 75-14084-00 | Replacement UV Bulb (302 nm) |
| 91-12999-00 | Digital Thermal Grayscale USB Printer |
| 73-13845-00 | UV Blocking Cover |
| UWS-500-8 | UV Transmitting Work Surface |
| 73-12593-00 | Trans Blue Conversion Screen |
Explore Automated Western Blot Analysis
Looking to improve your results? Evolve Your Protein Analysis with Simple Western Systems
Switch from the FluorChem Imagers to Simple Western and improve your protein detection. Simple Western instruments are the only gel-free, blot-free, and hands-free capillary-based western platform that integrate and automate the entire protein separation and detection process. Just load 3 µL of sample come back 3 hours later to highly quantitative and reproducible protein expression analysis using conventional Western blot antibodies. Replace your outdated Western blot imaging system with Simple Western with industry-leading IR/NIR fluorescence and chemiluminescence detection.
Comparison between FluorChem and Simple Western
| FluorChem Imagers | Simple Western Instruments | |
|---|---|---|
| NIR/IR detection limit | high pg | low pg |
| Chemi detection limit | high pg | low pg |
| Serial immunoassays | strip and re-probe | fully automated with RePlex™ |
| Multiplex across Chemi, IR, and NIR detection at once | no | yes |
| Single-step total protein detection and normalization | no | yes |
Fast and fully automated
With Simple Western Systems, it’s pipette, run and done! Simply load your sample, antibodies and reagents into the plate, insert your plate and cartridge into the Simple Western Instrument and press start. With as little as 3 hours of hands-free run time, you can be analyzing data and making decisions with your data in no time.
Reproducible
By eliminating the variability from manual steps of traditional western blotting protocols including sample loading, gel-to membrane transfer of proteins, and user and site differences, Simple Western technology is highly reproducible offering.
Quantitative
With Simple Western Systems, true protein quantitation is possible. With 25 or 100 independent sample throughput per run, Simple Western enables inclusion of standard curves, QC standards, and controls to design robust fully quantitative protein assays.
Bridge the Gap: Image Traditional Western Blots with Jess
Did you know Jess can image traditional Western blots?
Bring Jess into your lab and you can still image traditional chemiluminescent Western blots while you design your new fully quantitative and fully automated Simple Western experiments! Jess has a built-in chemiluminescence imager for Western blot imaging.
Resources to learn more about Simple Western
Discover more and solve your protein analysis problems with Simple Western. Read this comprehensive brochure on Simple Western Products to find out more.
Simple Western is an open platform, meaning the possibilities are almost endless when selecting antibodies. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test.
See the advantages of Simple Western against traditional western blotting in this comparative analysis.
Since its introduction Simple Western Technology has been trusted by thousands of laboratories around the globe. Search through over 2,800 publications where Simple Western Technology was used.