A20/TNFAIP3 Antibody Blocking Peptide
Novus Biologicals | Catalog # NBP1-77351PEP
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Key Product Details
Source
Synthetic
Applications
Antibody Competition
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Product Specifications
Description
A blocking peptide containing 14 amino acids near the carboxy terminus of human TNFAIP3.
Source: Synthetic
(Accession #: Q60769)
Application Notes
This peptide is useful as a blocking peptide for NBP1-77351.
For further blocking peptide related protocol, click here.
For further blocking peptide related protocol, click here.
Protein / Peptide Type
Antibody Blocking Peptide
Formulation, Preparation, and Storage
NBP1-77351PEP
| Formulation | PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin. |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: A20/TNFAIP3
A20/TNFAIP3 is a highly conserved protein sharing >90% amino acid sequence identity across various mammalian species and is highly expressed in T-cell and B-cells. Coding and non-coding single nucleotide polymorphisms (SNPs) of A20/TNFAIP3 have been associated with multiple autoinflammatory and autoimmune diseases including type 1 diabetes, rheumatoid arthritis, Crohn's disease, and systemic lupus erythematosus (SLE) (3). The two domains of A20/TNFAIP3 cooperate to regulate NF-kB signaling. Its N-terminal ovarian tumor (OTU) domain contains a catalytic cysteine (C103) which functions as a K63 deubiquitinase, whereas the 7 zinc fingers that make up the C-terminal domain mediate K48 polyubiquitination. To regulate NF-kB signaling, A20/TNFAIP3 removes K63-polyubiquitin chains from receptor-interacting protein 1 (RIP1) and NF-kB essential modulator (NEMO), thus preventing interactions with downstream partners. A20/TNFAIP3 also contributes to the degradation of RIP1 and Ubc13 through the addition of K48 polyubiquitin chains (4).
References
1.Dixit VM1, Green S, Sarma V, Holzman LB, Wolf FW, O'Rourke K, Ward PA, Prochownik EV, Marks RM. (1990) Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin. J Biol Chem. 265(5):2973-8. PMID: 2406243
2.Verstrepen L, Verhelst K, van Loo G, Carpentier I, Ley SC, Beyaert R. (2010) Expression, biological activities and mechanisms of action of A20 (TNFAIP3). Biochem Pharmacol. 80(12):2009-20. PMID: 20599425
3.Mele A, Cervantes JR, Chien V, Friedman D, Ferran C. (2014) Single nucleotide polymorphisms at the TNFAIP3/A20 locus and susceptibility/resistance to inflammatory and autoimmune diseases. Adv Exp Med Biol. 809:163-83. PMID: 25302371
4.Das T, Chen Z, Hendriks RW, Kool M. (2018) A20/Tumor Necrosis Factor alpha-Induced Protein 3 in Immune Cells Controls Development of Autoinflammation and Autoimmunity: Lessons from Mouse Models. Front Immunol. 9:104. PMID: 29515565
Alternate Names
OTUD7C, TNFA1P2, TNFAIP3
Gene Symbol
TNFAIP3
Additional A20/TNFAIP3 Products
Product Documents for A20/TNFAIP3 Antibody Blocking Peptide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for A20/TNFAIP3 Antibody Blocking Peptide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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FAQs for A20/TNFAIP3 Antibody Blocking Peptide
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Q: As written in immunogen on the datasheet, "the peptide was post-synthetically modified and conjugated to carrier protein" I thought this peptide could use for immunization. But is this peptide not attached carrier protein?
A: The immunogen used to create the antibody NBP1-77424 was conjugated to a carrier protein. The peptide is simply just a blocking peptide.
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Q: Following cleavage of A20/TNFAIP3, two full length fragments are released, one with 37 kDa and other one was approx. at 52 kDa. There are mentioning of 75Kda fragment and 37 KDa fragment in scientific papers but not 52 KDa. What is the significance of that specific band?
A: There is data available that explains 52 KDa band in A20/TNFAIP3. Using this antibody in Western Blot will result multiple bands. In LPS treatment, 52 KDa putative cleavage band was observed; however, the same band was not found in untreated THP1 cells.
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Q: As written in immunogen on the datasheet, "the peptide was post-synthetically modified and conjugated to carrier protein" I thought this peptide could use for immunization. But is this peptide not attached carrier protein?
A: The immunogen used to create the antibody NBP1-77424 was conjugated to a carrier protein. The peptide is simply just a blocking peptide.
-
Q: Following cleavage of A20/TNFAIP3, two full length fragments are released, one with 37 kDa and other one was approx. at 52 kDa. There are mentioning of 75Kda fragment and 37 KDa fragment in scientific papers but not 52 KDa. What is the significance of that specific band?
A: There is data available that explains 52 KDa band in A20/TNFAIP3. Using this antibody in Western Blot will result multiple bands. In LPS treatment, 52 KDa putative cleavage band was observed; however, the same band was not found in untreated THP1 cells.
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