Absolute Rainbow Cell Count Particle Set

Novus Biologicals | Catalog # NBP3-00495

Novus Biologicals
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Product Specifications

Description

The Absolute Rainbow Cell Count Particles are designed as reference particles with a known number of particles per mL for counting the absolute cell number in flow cytometry. These fluorescent particles can be detected in FITC, PE, PE-TR, PE-Cy5, and APC channels. Concentration: 10^6 particles / mL

Particle size: 8.0 - 12.9 micron

Particle material: polystyrene

Application Notes

Shake bottle vigorously or vortex briefly before use.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

Scientific Data Images for Absolute Rainbow Cell Count Particle Set

Absolute Rainbow Cell Count Particle Set [NBP3-00495] - Histograms showing counts of particles in the Orange, Red, and Green channels.

Formulation, Preparation, and Storage

NBP3-00495
Formulation 0.016 M PBS (pH 7.4), 0.2% BSA
Preservative 0.2% Sodium Azide
Concentration Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C in the dark. Do not freeze.

Background: Absolute Rainbow Cell Count Particle Set

Alternate Names

Absolute Counting Beads, Count Beads, Counting Beads, Flow Cytometry Counting Beads

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Product Documents for Absolute Rainbow Cell Count Particle Set

Certificate of Analysis

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Product Specific Notices for Absolute Rainbow Cell Count Particle Set

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Protocols

View specific protocols for Absolute Rainbow Cell Count Particle Set (NBP3-00495):

Shake bottle vigorously or vortex briefly before use.
1. Add the monoclonal antibody of your choice to 100 uL of test sample.
2. Incubate, lyse, wash, and then resuspend in 1 to 2 mL PBS. If staining and lysing are not needed, just mix a known volume of test sample in 1 to 2 mL PBS
3. Add exactly 50 uL of NBP3-00495 particles to the suspension. Precision when pipetting is absolutely critical.
4. Run the sample in the flow cytometer and obtain the amount of events for the NBP3-00495 particles and your test sample.
5. Calculate the number of cells accordingly:
(A/B) x (C/D) = Number of cells per uL
where
A = number of events for the test sample
B = number of events for the NBP3-00495 particles
C = number of NBP3-00495 particles per 50 uL
D = volume of test sample initally used

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